Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 82911..83638 Replicon plasmid unnamed1
Accession NZ_CP081725
Organism Escherichia coli strain CX07

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag J6O12_RS24685 Protein ID WP_000558568.1
Coordinates 82911..83222 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag J6O12_RS24690 Protein ID WP_000990392.1
Coordinates 83219..83638 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O12_RS24655 (J6O12_24570) 78233..78930 + 698 WP_223335778.1 IS1 family transposase -
J6O12_RS24660 (J6O12_24575) 78940..80022 + 1083 WP_032140505.1 lytic murein transglycosylase B -
J6O12_RS24665 (J6O12_24580) 80130..80987 - 858 Protein_82 APC family permease -
J6O12_RS24670 (J6O12_24585) 81040..81744 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O12_RS24675 (J6O12_24590) 81809..82270 + 462 Protein_84 lactate utilization protein C -
J6O12_RS24680 (J6O12_24595) 82320..82700 - 381 Protein_85 gluconate permease -
J6O12_RS24685 (J6O12_24600) 82911..83222 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O12_RS24690 (J6O12_24605) 83219..83638 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
J6O12_RS24700 85082..85282 - 201 Protein_89 hypothetical protein -
J6O12_RS24705 (J6O12_24620) 85444..85794 + 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
J6O12_RS24710 (J6O12_24625) 86099..86575 - 477 WP_000043177.1 Dps family protein -
J6O12_RS24715 (J6O12_24630) 86699..87646 - 948 Protein_92 transposase -
J6O12_RS24720 (J6O12_24635) 87712..88416 + 705 WP_088765929.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146661 146661
- inside IScluster/Tn - - 78427..89759 11332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T213263 WP_000558568.1 NZ_CP081725:82911-83222 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T213263 NZ_CP107506:c4371-4057 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT213263 WP_000990392.1 NZ_CP081725:83219-83638 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT213263 NZ_CP107506:c4637-4359 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References