Detailed information of TA system TA213245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1961247..1961749
• Replicon: chromosome
• Accession: NZ_CP081724
• Organism: Escherichia coli strain CX07

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: J6O12_RS09440
• Protein ID: WP_000767829.1
• Coordinates: 1961495..1961749 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: J6O12_RS09435
• Protein ID: WP_001259255.1
• Coordinates: 1961247..1961498 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O12_RS09410 (1956425)	1956425..1957492	-	1068	WP_000080142.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
J6O12_RS09415 (1957492)	1957492..1958562	-	1071	WP_032248775.1	histidinol-phosphate transaminase	-
J6O12_RS09420 (1958559)	1958559..1959863	-	1305	WP_016240067.1	histidinol dehydrogenase	-
J6O12_RS09425 (1959869)	1959869..1960768	-	900	WP_000131786.1	ATP phosphoribosyltransferase	-
J6O12_RS09430 (1960914)	1960914..1960964	-	51	WP_001364200.1	his operon leader peptide	-
J6O12_RS09435 (1961247)	1961247..1961498	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
J6O12_RS09440 (1961495)	1961495..1961749	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
J6O12_RS09445 (1961832)	1961832..1962656	+	825	WP_000754749.1	SDR family oxidoreductase	-
J6O12_RS09450 (1962702)	1962702..1963631	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
J6O12_RS09455 (1963846)	1963846..1963908	+	63	WP_010723108.1	membrane protein YoeI	-
J6O12_RS09460 (1963898)	1963898..1965256	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
J6O12_RS09465 (1965435)	1965435..1966493	+	1059	WP_000492297.1	thiosulfate utilization transporter TsuA/YeeE	-
J6O12_RS09470 (1966507)	1966507..1966734	+	228	WP_000985279.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T213245 WP_000767829.1 NZ_CP081724:1961495-1961749 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T213245 NZ_CP107500:c91487-91071 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT213245 WP_001259255.1 NZ_CP081724:1961247-1961498 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT213245 NZ_CP107500:c91714-91484 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0