Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1164032..1164759 Replicon chromosome
Accession NZ_CP081724
Organism Escherichia coli strain CX07

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag J6O12_RS05700 Protein ID WP_000547564.1
Coordinates 1164032..1164343 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J6O12_RS05705 Protein ID WP_000126294.1
Coordinates 1164340..1164759 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O12_RS05670 (1159174) 1159174..1160883 + 1710 WP_001288140.1 formate hydrogenlyase subunit HycE -
J6O12_RS05675 (1160893) 1160893..1161435 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
J6O12_RS05680 (1161435) 1161435..1162202 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
J6O12_RS05685 (1162199) 1162199..1162609 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
J6O12_RS05690 (1162602) 1162602..1163072 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
J6O12_RS05695 (1163097) 1163097..1163858 + 762 WP_001026446.1 hypothetical protein -
J6O12_RS05700 (1164032) 1164032..1164343 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O12_RS05705 (1164340) 1164340..1164759 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
J6O12_RS05710 (1164873) 1164873..1166297 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
J6O12_RS05715 (1166306) 1166306..1167763 - 1458 WP_001107882.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
J6O12_RS05720 (1168023) 1168023..1169033 + 1011 WP_001402444.1 DNA-binding transcriptional regulator AscG -
J6O12_RS05725 (1169182) 1169182..1169709 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1118516..1165193 46677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T213244 WP_000547564.1 NZ_CP081724:1164032-1164343 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T213244 NZ_CP107500:c78398-78249 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT213244 WP_000126294.1 NZ_CP081724:1164340-1164759 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT213244 NZ_CP107500:78443-78502 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References