Detailed information of TA system TA213241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 819736..820429
• Replicon: chromosome
• Accession: NZ_CP081724
• Organism: Escherichia coli strain CX07

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: J6O12_RS04075
• Protein ID: WP_000415584.1
• Coordinates: 819736..820032 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: J6O12_RS04080
• Protein ID: WP_000650107.1
• Coordinates: 820034..820429 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O12_RS04040 (814870)	814870..815349	+	480	WP_000065333.1	Hcp family type VI secretion system effector	-
J6O12_RS04045 (815352)	815352..816062	+	711	WP_000834029.1	hypothetical protein	-
J6O12_RS04050 (816069)	816069..816401	+	333	WP_000914691.1	DUF2645 family protein	-
J6O12_RS04055 (816447)	816447..817796	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
J6O12_RS04060 (817793)	817793..818452	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
J6O12_RS04065 (818604)	818604..818996	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
J6O12_RS04070 (819049)	819049..819531	+	483	WP_000609258.1	GyrI-like domain-containing protein	-
J6O12_RS04075 (819736)	819736..820032	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
J6O12_RS04080 (820034)	820034..820429	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
J6O12_RS04085 (820562)	820562..822169	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
J6O12_RS04090 (822307)	822307..824565	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T213241 WP_000415584.1 NZ_CP081724:819736-820032 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T213241 NZ_CP107499:c49368-48886 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT213241 WP_000650107.1 NZ_CP081724:820034-820429 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT213241 NZ_CP107499:c49622-49356 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65