Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 661158..661957 Replicon chromosome
Accession NZ_CP081724
Organism Escherichia coli strain CX07

Toxin (Protein)


Gene name yhaV Uniprot ID D7XZ20
Locus tag J6O12_RS03260 Protein ID WP_000347264.1
Coordinates 661158..661622 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J6O12_RS03265 Protein ID WP_001307405.1
Coordinates 661622..661957 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O12_RS03230 (656159) 656159..656593 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J6O12_RS03235 (656611) 656611..657489 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
J6O12_RS03240 (657479) 657479..658258 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
J6O12_RS03245 (658269) 658269..658742 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J6O12_RS03250 (658765) 658765..660045 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J6O12_RS03255 (660294) 660294..661103 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J6O12_RS03260 (661158) 661158..661622 - 465 WP_000347264.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J6O12_RS03265 (661622) 661622..661957 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J6O12_RS03270 (662106) 662106..663677 - 1572 WP_001273753.1 galactarate dehydratase -
J6O12_RS03275 (664052) 664052..665386 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J6O12_RS03280 (665402) 665402..666172 + 771 WP_001058207.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17894.29 Da        Isoelectric Point: 9.4945

>T213238 WP_000347264.1 NZ_CP081724:c661622-661158 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFDAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T213238 NZ_CP107498:3578859-3579266 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT213238 WP_001307405.1 NZ_CP081724:c661957-661622 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT213238 NZ_CP107498:3578612-3578878 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QML2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References