Detailed information of TA system TA213233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 101828..102555
• Replicon: plasmid unnamed1
• Accession: NZ_CP081720
• Organism: Escherichia coli strain CX05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: J6O38_RS24705
• Protein ID: WP_000558568.1
• Coordinates: 102244..102555 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: J6O38_RS24700
• Protein ID: WP_000990392.1
• Coordinates: 101828..102247 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O38_RS24670 (J6O38_24590)	97050..97754	-	705	WP_088765929.1	IS6-like element IS26 family transposase	-
J6O38_RS24675 (J6O38_24595)	97820..98767	+	948	Protein_84	transposase	-
J6O38_RS24680 (J6O38_24600)	98891..99367	+	477	WP_000043177.1	Dps family protein	-
J6O38_RS24685 (J6O38_24605)	99672..100022	-	351	WP_000928911.1	metalloregulator ArsR/SmtB family transcription factor	-
J6O38_RS24690	100184..100384	+	201	Protein_87	hypothetical protein	-
J6O38_RS24695 (J6O38_24615)	100640..101760	+	1121	WP_088765928.1	IS3 family transposase	-
J6O38_RS24700 (J6O38_24620)	101828..102247	-	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
J6O38_RS24705 (J6O38_24625)	102244..102555	-	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6O38_RS24710 (J6O38_24630)	102766..103146	+	381	Protein_91	gluconate permease	-
J6O38_RS24715 (J6O38_24635)	103196..103657	-	462	Protein_92	lactate utilization protein C	-
J6O38_RS24720 (J6O38_24640)	103722..104426	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O38_RS24725 (J6O38_24645)	104479..105336	+	858	Protein_94	APC family permease	-
J6O38_RS24730 (J6O38_24650)	105444..106526	-	1083	WP_032140505.1	lytic murein transglycosylase B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..146658	146658
	-	inside	IScluster/Tn	-	-	95707..107039	11332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T213233 WP_000558568.1 NZ_CP081720:c102555-102244 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T213233 NZ_CP107498:c2143781-2143497 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT213233 WP_000990392.1 NZ_CP081720:c102247-101828 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT213233 NZ_CP107498:c2144013-2143771 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4