Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 101828..102555 Replicon plasmid unnamed1
Accession NZ_CP081720
Organism Escherichia coli strain CX05

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag J6O38_RS24705 Protein ID WP_000558568.1
Coordinates 102244..102555 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag J6O38_RS24700 Protein ID WP_000990392.1
Coordinates 101828..102247 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O38_RS24670 (J6O38_24590) 97050..97754 - 705 WP_088765929.1 IS6-like element IS26 family transposase -
J6O38_RS24675 (J6O38_24595) 97820..98767 + 948 Protein_84 transposase -
J6O38_RS24680 (J6O38_24600) 98891..99367 + 477 WP_000043177.1 Dps family protein -
J6O38_RS24685 (J6O38_24605) 99672..100022 - 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
J6O38_RS24690 100184..100384 + 201 Protein_87 hypothetical protein -
J6O38_RS24695 (J6O38_24615) 100640..101760 + 1121 WP_088765928.1 IS3 family transposase -
J6O38_RS24700 (J6O38_24620) 101828..102247 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
J6O38_RS24705 (J6O38_24625) 102244..102555 - 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O38_RS24710 (J6O38_24630) 102766..103146 + 381 Protein_91 gluconate permease -
J6O38_RS24715 (J6O38_24635) 103196..103657 - 462 Protein_92 lactate utilization protein C -
J6O38_RS24720 (J6O38_24640) 103722..104426 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O38_RS24725 (J6O38_24645) 104479..105336 + 858 Protein_94 APC family permease -
J6O38_RS24730 (J6O38_24650) 105444..106526 - 1083 WP_032140505.1 lytic murein transglycosylase B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..146658 146658
- inside IScluster/Tn - - 95707..107039 11332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T213233 WP_000558568.1 NZ_CP081720:c102555-102244 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T213233 NZ_CP107498:c2143781-2143497 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT213233 WP_000990392.1 NZ_CP081720:c102247-101828 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT213233 NZ_CP107498:c2144013-2143771 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References