Detailed information of TA system TA213213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1164035..1164762
• Replicon: chromosome
• Accession: NZ_CP081719
• Organism: Escherichia coli strain CX05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: J6O38_RS05700
• Protein ID: WP_000547564.1
• Coordinates: 1164035..1164346 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6O38_RS05705
• Protein ID: WP_000126294.1
• Coordinates: 1164343..1164762 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O38_RS05670 (1159177)	1159177..1160886	+	1710	WP_001288140.1	formate hydrogenlyase subunit HycE	-
J6O38_RS05675 (1160896)	1160896..1161438	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
J6O38_RS05680 (1161438)	1161438..1162205	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
J6O38_RS05685 (1162202)	1162202..1162612	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
J6O38_RS05690 (1162605)	1162605..1163075	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
J6O38_RS05695 (1163100)	1163100..1163861	+	762	WP_001026446.1	hypothetical protein	-
J6O38_RS05700 (1164035)	1164035..1164346	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6O38_RS05705 (1164343)	1164343..1164762	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J6O38_RS05710 (1164876)	1164876..1166300	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
J6O38_RS05715 (1166309)	1166309..1167766	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J6O38_RS05720 (1168026)	1168026..1169036	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
J6O38_RS05725 (1169185)	1169185..1169712	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1118519..1165196	46677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T213213 WP_000547564.1 NZ_CP081719:1164035-1164346 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T213213 NZ_CP107492:1088-1354 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT213213 WP_000126294.1 NZ_CP081719:1164343-1164762 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT213213 NZ_CP107492:880-1104 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5