Detailed information of TA system TA213170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3700401..3701095
• Replicon: chromosome
• Accession: NZ_CP081711
• Organism: Escherichia coli strain AH21

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: J6O42_RS17900
• Protein ID: WP_001263489.1
• Coordinates: 3700401..3700799 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: J6O42_RS17905
• Protein ID: WP_000554758.1
• Coordinates: 3700802..3701095 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3695989)	3695989..3696069	-	81	NuclAT_12	-	-
- (3695989)	3695989..3696069	-	81	NuclAT_12	-	-
- (3695989)	3695989..3696069	-	81	NuclAT_12	-	-
- (3695989)	3695989..3696069	-	81	NuclAT_12	-	-
J6O42_RS17875 (3696665)	3696665..3697123	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J6O42_RS17880 (3697384)	3697384..3698841	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
J6O42_RS17885 (3698898)	3698898..3699419	-	522	Protein_3494	peptide chain release factor H	-
J6O42_RS17890 (3699415)	3699415..3699621	-	207	Protein_3495	RtcB family protein	-
J6O42_RS17895 (3699939)	3699939..3700391	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
J6O42_RS17900 (3700401)	3700401..3700799	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J6O42_RS17905 (3700802)	3700802..3701095	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J6O42_RS17910 (3701147)	3701147..3702202	-	1056	WP_001226164.1	DNA polymerase IV	-
J6O42_RS17915 (3702273)	3702273..3703196	-	924	WP_032191601.1	putative lateral flagellar export/assembly protein LafU	-
J6O42_RS17920 (3703199)	3703199..3704062	-	864	WP_223597172.1	flagellar motor stator protein MotA	-
J6O42_RS17925 (3704075)	3704075..3704791	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
J6O42_RS17930 (3704811)	3704811..3705278	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T213170 WP_001263489.1 NZ_CP081711:c3700799-3700401 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T213170 NZ_CP107478:c4904160-4903876 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT213170 WP_000554758.1 NZ_CP081711:c3701095-3700802 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT213170 NZ_CP107478:c4904392-4904150 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3