Detailed information of TA system TA213147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3828569..3829263
• Replicon: chromosome
• Accession: NZ_CP081706
• Organism: Escherichia coli strain AH04

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: J6O21_RS19155
• Protein ID: WP_001263493.1
• Coordinates: 3828569..3828967 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: J6O21_RS19160
• Protein ID: WP_001399937.1
• Coordinates: 3828970..3829263 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3824156)	3824156..3824236	-	81	NuclAT_12	-	-
- (3824156)	3824156..3824236	-	81	NuclAT_12	-	-
- (3824156)	3824156..3824236	-	81	NuclAT_12	-	-
- (3824156)	3824156..3824236	-	81	NuclAT_12	-	-
J6O21_RS19130 (3824832)	3824832..3825290	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J6O21_RS19135 (3825551)	3825551..3827008	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
J6O21_RS19140 (3827065)	3827065..3827586	-	522	Protein_3745	peptide chain release factor H	-
J6O21_RS19145 (3827582)	3827582..3827788	-	207	Protein_3746	RtcB family protein	-
J6O21_RS19150 (3828107)	3828107..3828559	-	453	WP_072146325.1	GNAT family N-acetyltransferase	-
J6O21_RS19155 (3828569)	3828569..3828967	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J6O21_RS19160 (3828970)	3828970..3829263	-	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J6O21_RS19165 (3829315)	3829315..3830370	-	1056	WP_001226161.1	DNA polymerase IV	-
J6O21_RS19170 (3830441)	3830441..3831226	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
J6O21_RS19175 (3831198)	3831198..3832910	+	1713	Protein_3752	flagellar biosynthesis protein FlhA	-
J6O21_RS19180 (3833228)	3833228..3833725	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3759103..3836429	77326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T213147 WP_001263493.1 NZ_CP081706:c3828967-3828569 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T213147 NZ_CP107473:1458689-1458907 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT213147 WP_001399937.1 NZ_CP081706:c3829263-3828970 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT213147 NZ_CP107473:1458288-1458662 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1EXB8