Detailed information of TA system TA213127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 598389..599188
• Replicon: chromosome
• Accession: NZ_CP081706
• Organism: Escherichia coli strain AH04

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: J6O21_RS02955
• Protein ID: WP_000347267.1
• Coordinates: 598389..598853 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: J6O21_RS02960
• Protein ID: WP_001302819.1
• Coordinates: 598853..599188 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O21_RS02925 (593390)	593390..593824	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
J6O21_RS02930 (593842)	593842..594720	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
J6O21_RS02935 (594710)	594710..595489	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
J6O21_RS02940 (595500)	595500..595973	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
J6O21_RS02945 (595996)	595996..597276	-	1281	WP_000681903.1	tagatose-bisphosphate aldolase subunit KbaZ	-
J6O21_RS02950 (597525)	597525..598334	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
J6O21_RS02955 (598389)	598389..598853	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
J6O21_RS02960 (598853)	598853..599188	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
J6O21_RS02965 (599337)	599337..600908	-	1572	WP_057107993.1	galactarate dehydratase	-
J6O21_RS02970 (601283)	601283..602617	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
J6O21_RS02975 (602633)	602633..603403	+	771	WP_001559981.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T213127 WP_000347267.1 NZ_CP081706:c598853-598389 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T213127 NZ_CP107466:c4371-4057 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT213127 WP_001302819.1 NZ_CP081706:c599188-598853 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT213127 NZ_CP107466:c4637-4359 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4