Detailed information of TA system TA213115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3828570..3829264
• Replicon: chromosome
• Accession: NZ_CP081701
• Organism: Escherichia coli strain AH02

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: J6O02_RS19155
• Protein ID: WP_001263493.1
• Coordinates: 3828570..3828968 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: J6O02_RS19160
• Protein ID: WP_001399937.1
• Coordinates: 3828971..3829264 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3824157)	3824157..3824237	-	81	NuclAT_12	-	-
- (3824157)	3824157..3824237	-	81	NuclAT_12	-	-
- (3824157)	3824157..3824237	-	81	NuclAT_12	-	-
- (3824157)	3824157..3824237	-	81	NuclAT_12	-	-
J6O02_RS19130 (3824833)	3824833..3825291	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
J6O02_RS19135 (3825552)	3825552..3827009	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
J6O02_RS19140 (3827066)	3827066..3827587	-	522	Protein_3747	peptide chain release factor H	-
J6O02_RS19145 (3827583)	3827583..3827789	-	207	Protein_3748	RtcB family protein	-
J6O02_RS19150 (3828108)	3828108..3828560	-	453	WP_072146325.1	GNAT family N-acetyltransferase	-
J6O02_RS19155 (3828570)	3828570..3828968	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
J6O02_RS19160 (3828971)	3828971..3829264	-	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
J6O02_RS19165 (3829316)	3829316..3830371	-	1056	WP_001226161.1	DNA polymerase IV	-
J6O02_RS19170 (3830442)	3830442..3831227	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
J6O02_RS19175 (3831199)	3831199..3832911	+	1713	Protein_3754	flagellar biosynthesis protein FlhA	-
J6O02_RS19180 (3833229)	3833229..3833726	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3759104..3836430	77326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T213115 WP_001263493.1 NZ_CP081701:c3828968-3828570 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T213115 NZ_CP107463:355289-355636 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT213115 WP_001399937.1 NZ_CP081701:c3829264-3828971 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT213115 NZ_CP107463:355636-355935 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: S1EXB8