Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 67532..68166 Replicon chromosome
Accession NZ_CP081701
Organism Escherichia coli strain AH02

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag J6O02_RS00325 Protein ID WP_057108200.1
Coordinates 67873..68166 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J6O02_RS00320 Protein ID WP_057108202.1
Coordinates 67532..67858 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O02_RS00300 (62653) 62653..63258 - 606 Protein_58 DNA damage-inducible protein D -
J6O02_RS00310 (64027) 64027..66624 - 2598 WP_223566610.1 DEAD/DEAH box helicase -
J6O02_RS00315 (66614) 66614..67462 - 849 WP_057108201.1 SAVED domain-containing protein -
J6O02_RS00320 (67532) 67532..67858 - 327 WP_057108202.1 HigA family addiction module antitoxin Antitoxin
J6O02_RS00325 (67873) 67873..68166 - 294 WP_057108200.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J6O02_RS00330 (68296) 68296..69507 - 1212 WP_057108199.1 site-specific integrase -
J6O02_RS00335 (69679) 69679..70542 - 864 WP_000621340.1 YicC/YloC family endoribonuclease -
J6O02_RS00340 (70669) 70669..71385 + 717 WP_001247093.1 ribonuclease PH -
J6O02_RS00345 (71450) 71450..72091 + 642 WP_000806161.1 orotate phosphoribosyltransferase -
J6O02_RS00350 (72128) 72128..72724 - 597 WP_000818601.1 nucleoid occlusion factor SlmA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11470.89 Da        Isoelectric Point: 5.1406

>T213087 WP_057108200.1 NZ_CP081701:c68166-67873 [Escherichia coli]
MAVEFRDTWLEAFYEDDQNHRRIPSTIEGALFRKLEILDAATQESDLRIPPGNRFEHLEGNMAGWCSIRVNKQYRLIFRW
IDGVAQDTYLDPHVYRG

Download         Length: 294 bp

>T213087 NZ_CP107452:3077099-3077467 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12363.29 Da        Isoelectric Point: 8.8778

>AT213087 WP_057108202.1 NZ_CP081701:c67858-67532 [Escherichia coli]
MRNTKRRPVTVGQMLVTEFLEPMNIEINELAEAMGVHRNTLSRIVHDKGALTAPMSIKLAAALGNTPEFWLNIQHSVELW
DVRHRAFEQEAKNVKRVTPHLENREAVI

Download         Length: 327 bp

>AT213087 NZ_CP107452:3076881-3077102 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References