Detailed information of TA system TA213069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1098880..1099607
• Replicon: chromosome
• Accession: NZ_CP081698
• Organism: Escherichia coli strain CT02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: J6N91_RS05320
• Protein ID: WP_000547564.1
• Coordinates: 1098880..1099191 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: J6N91_RS05325
• Protein ID: WP_000126294.1
• Coordinates: 1099188..1099607 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6N91_RS05290 (1094022)	1094022..1095731	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
J6N91_RS05295 (1095741)	1095741..1096283	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
J6N91_RS05300 (1096283)	1096283..1097050	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
J6N91_RS05305 (1097047)	1097047..1097457	+	411	WP_223602094.1	formate hydrogenlyase assembly protein HycH	-
J6N91_RS05310 (1097450)	1097450..1097920	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
J6N91_RS05315 (1097945)	1097945..1098706	+	762	WP_042202360.1	hypothetical protein	-
J6N91_RS05320 (1098880)	1098880..1099191	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
J6N91_RS05325 (1099188)	1099188..1099607	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
J6N91_RS05330 (1099721)	1099721..1101145	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
J6N91_RS05335 (1101154)	1101154..1102611	-	1458	WP_001107882.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
J6N91_RS05340 (1102871)	1102871..1103881	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
J6N91_RS05345 (1104030)	1104030..1104557	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1059671..1121434	61763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T213069 WP_000547564.1 NZ_CP081698:1098880-1099191 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T213069 NZ_CP107447:4189342-4189560 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT213069 WP_000126294.1 NZ_CP081698:1099188-1099607 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT213069 NZ_CP107447:4188941-4189315 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5