Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 626011..626810 Replicon chromosome
Accession NZ_CP081698
Organism Escherichia coli strain CT02

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag J6N91_RS03040 Protein ID WP_000347273.1
Coordinates 626011..626475 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J6N91_RS03045 Protein ID WP_001307405.1
Coordinates 626475..626810 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6N91_RS03010 (621012) 621012..621446 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J6N91_RS03015 (621464) 621464..622342 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
J6N91_RS03020 (622332) 622332..623111 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
J6N91_RS03025 (623122) 623122..623595 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
J6N91_RS03030 (623618) 623618..624898 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J6N91_RS03035 (625147) 625147..625956 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J6N91_RS03040 (626011) 626011..626475 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J6N91_RS03045 (626475) 626475..626810 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J6N91_RS03050 (626959) 626959..628530 - 1572 WP_001273753.1 galactarate dehydratase -
J6N91_RS03055 (628905) 628905..630239 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J6N91_RS03060 (630255) 630255..631025 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T213065 WP_000347273.1 NZ_CP081698:c626475-626011 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T213065 NZ_CP107447:353992-354339 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT213065 WP_001307405.1 NZ_CP081698:c626810-626475 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT213065 NZ_CP107447:354339-354638 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References