Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3076..3803 Replicon plasmid unnamed3
Accession NZ_CP081693
Organism Escherichia coli strain 0H25

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag J6O25_RS26655 Protein ID WP_000558568.1
Coordinates 3076..3387 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag J6O25_RS26660 Protein ID WP_000990392.1
Coordinates 3384..3803 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O25_RS26635 (J6O25_26500) 936..1181 - 246 WP_223598065.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
J6O25_RS26640 (J6O25_26505) 1257..1961 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O25_RS26645 (J6O25_26510) 2025..2435 + 411 Protein_3 lactate utilization protein C -
J6O25_RS26650 (J6O25_26515) 2485..2865 - 381 Protein_4 gluconate permease -
J6O25_RS26655 (J6O25_26520) 3076..3387 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O25_RS26660 (J6O25_26525) 3384..3803 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
J6O25_RS26670 5247..5448 - 202 Protein_8 hypothetical protein -
J6O25_RS26675 (J6O25_26540) 5610..5960 + 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
J6O25_RS26680 (J6O25_26545) 6265..6741 - 477 WP_000043177.1 Dps family protein -
J6O25_RS26685 (J6O25_26550) 6865..7813 - 949 Protein_11 transposase -
J6O25_RS26690 (J6O25_26555) 7879..8583 + 705 WP_088765929.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..69249 69249
- inside IScluster/Tn - - 1257..16815 15558


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T213063 WP_000558568.1 NZ_CP081693:3076-3387 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T213063 NZ_CP107446:2292-2558 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT213063 WP_000990392.1 NZ_CP081693:3384-3803 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT213063 NZ_CP107446:2084-2308 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References