Detailed information of TA system TA021305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3359680..3360278
• Replicon: chromosome
• Accession: NC_007517
• Organism: Geobacter metallireducens GS-15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q39RD6
• Locus tag: GMET_RS14910
• Protein ID: WP_004512906.1
• Coordinates: 3360000..3360278 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GMET_RS14905
• Protein ID: WP_004512905.1
• Coordinates: 3359680..3359985 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMET_RS14880	3355168..3356538	-	1371	WP_011366129.1	hypothetical protein	-
GMET_RS14885	3356656..3356997	-	342	WP_004512901.1	hypothetical protein	-
GMET_RS14890	3356999..3357280	-	282	WP_035468656.1	hypothetical protein	-
GMET_RS14895	3357345..3358025	-	681	WP_004512903.1	class I SAM-dependent methyltransferase	-
GMET_RS14900	3358146..3359660	+	1515	WP_004512904.1	B12-binding domain-containing radical SAM protein	-
GMET_RS14905	3359680..3359985	-	306	WP_004512905.1	HigA family addiction module antidote protein	Antitoxin
GMET_RS14910	3360000..3360278	-	279	WP_004512906.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GMET_RS14915	3360327..3362684	-	2358	WP_004512907.1	endonuclease MutS2	-
GMET_RS14920	3362747..3363703	-	957	WP_004513407.1	IS110-like element ISGme8 family transposase	-
GMET_RS14925	3364019..3364384	-	366	WP_004514467.1	peptide chain release factor-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3362747..3363703	956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10890.37 Da
• Isoelectric Point: 8.2868

>T21305 WP_004512906.1 NC_007517:c3360278-3360000 [Geobacter metallireducens GS-15]
MIKSFRCKDTQALFEGKCPRRFKAFSTVAERKLQMLDDAHSPEDLRSPPGNRLEVLRGDRKGQWSIRINDQWRLCFRFED
GNAFDVEIVDYH

Nucleotide

• Length: 279 bp

>T21305 NC_007517:c3360278-3360000 [Geobacter metallireducens GS-15]
ATGATCAAGTCATTCCGCTGCAAGGACACCCAGGCTTTGTTCGAGGGGAAATGCCCGCGTCGGTTCAAGGCGTTCTCTAC
GGTTGCCGAGCGAAAGCTGCAAATGCTTGATGACGCCCACTCGCCCGAAGATCTCCGCTCGCCCCCGGGCAACCGCCTGG
AAGTCTTGCGCGGTGACCGCAAAGGACAGTGGAGCATCCGGATCAACGATCAGTGGCGCCTTTGCTTTCGCTTTGAGGAT
GGCAACGCCTTTGATGTGGAGATCGTGGATTACCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11614.51 Da
• Isoelectric Point: 10.2920

>AT21305 WP_004512905.1 NC_007517:c3359985-3359680 [Geobacter metallireducens GS-15]
MNPTNRMRPIHPGEVLREEFLIPLGMSAHALALELKVPAPRINDIVRERRAVTPDTALRLARYFGTTPQFWLNLQTSYDL
KIAERELGSKIEREVHTRLVA

Nucleotide

• Length: 306 bp

>AT21305 NC_007517:c3359985-3359680 [Geobacter metallireducens GS-15]
ATGAACCCCACCAACAGAATGCGCCCCATTCATCCCGGTGAAGTGCTGCGTGAGGAGTTCCTGATCCCCCTGGGGATGAG
TGCCCATGCGCTGGCGCTGGAACTCAAGGTGCCGGCGCCGCGCATCAACGACATCGTGCGCGAGCGCCGTGCCGTTACGC
CCGACACGGCTCTCAGGCTGGCCCGGTACTTCGGCACAACGCCGCAGTTCTGGCTCAATCTGCAGACCAGTTACGACCTT
AAGATCGCCGAACGGGAACTGGGCTCGAAAATCGAGCGTGAAGTTCACACACGCCTCGTGGCCTAG

Structures

Toxin

• AlphaFold DB: Q39RD6