Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1968515..1969017 Replicon chromosome
Accession NZ_CP081690
Organism Escherichia coli strain 0H25

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag J6O25_RS09670 Protein ID WP_000767829.1
Coordinates 1968763..1969017 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag J6O25_RS09665 Protein ID WP_001259255.1
Coordinates 1968515..1968766 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O25_RS09640 (1963693) 1963693..1964760 - 1068 WP_000080066.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
J6O25_RS09645 (1964760) 1964760..1965830 - 1071 WP_000108961.1 histidinol-phosphate transaminase -
J6O25_RS09650 (1965827) 1965827..1967131 - 1305 WP_123282557.1 histidinol dehydrogenase -
J6O25_RS09655 (1967137) 1967137..1968036 - 900 WP_000131777.1 ATP phosphoribosyltransferase -
J6O25_RS09660 (1968182) 1968182..1968232 - 51 WP_001364200.1 his operon leader peptide -
J6O25_RS09665 (1968515) 1968515..1968766 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
J6O25_RS09670 (1968763) 1968763..1969017 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
J6O25_RS09675 (1969100) 1969100..1969924 + 825 WP_000754749.1 SDR family oxidoreductase -
J6O25_RS09680 (1969970) 1969970..1970752 + 783 Protein_1893 LysR substrate-binding domain-containing protein -
J6O25_RS09685 (1970759) 1970759..1971910 - 1152 WP_001254932.1 IS30-like element IS30 family transposase -
J6O25_RS09690 (1971975) 1971975..1972571 + 597 Protein_1895 IS3 family transposase -
J6O25_RS09695 (1972853) 1972853..1973929 + 1077 WP_000227970.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T213041 WP_000767829.1 NZ_CP081690:1968763-1969017 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T213041 NZ_CP107437:c125824-125408 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT213041 WP_001259255.1 NZ_CP081690:1968515-1968766 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT213041 NZ_CP107437:c126051-125821 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References