Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 19409..20136 Replicon plasmid unnamed3
Accession NZ_CP081687
Organism Escherichia coli strain 0H23

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag J6O16_RS26750 Protein ID WP_000558568.1
Coordinates 19409..19720 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag J6O16_RS26755 Protein ID WP_000990392.1
Coordinates 19717..20136 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O16_RS26715 (J6O16_26630) 14595..14906 + 312 WP_024191312.1 hypothetical protein -
J6O16_RS26720 (J6O16_26635) 14996..16081 - 1086 WP_001148851.1 iron-containing alcohol dehydrogenase family protein -
J6O16_RS26725 (J6O16_26640) 16115..17272 - 1158 WP_000217345.1 alpha-hydroxy acid oxidase -
J6O16_RS26730 (J6O16_26645) 17269..17514 - 246 WP_223598065.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
J6O16_RS26735 (J6O16_26650) 17590..18294 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O16_RS26740 (J6O16_26655) 18358..18768 + 411 Protein_24 lactate utilization protein C -
J6O16_RS26745 (J6O16_26660) 18818..19198 - 381 Protein_25 gluconate permease -
J6O16_RS26750 (J6O16_26665) 19409..19720 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
J6O16_RS26755 (J6O16_26670) 19717..20136 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
J6O16_RS26765 21580..21780 - 201 Protein_29 hypothetical protein -
J6O16_RS26770 (J6O16_26685) 21942..22292 + 351 WP_000928911.1 metalloregulator ArsR/SmtB family transcription factor -
J6O16_RS26775 (J6O16_26690) 22597..23073 - 477 WP_000043177.1 Dps family protein -
J6O16_RS26780 (J6O16_26695) 23197..24144 - 948 Protein_32 transposase -
J6O16_RS26785 (J6O16_26700) 24210..24914 + 705 WP_088765929.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..69240 69240
- inside IScluster/Tn - - 16..33146 33130


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T213033 WP_000558568.1 NZ_CP081687:19409-19720 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T213033 NZ_CP107436:2023859-2023961 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT213033 WP_000990392.1 NZ_CP081687:19717-20136 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT213033 NZ_CP107436:c2023968-2023823 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References