Detailed information of TA system TA213006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 775345..776038
• Replicon: chromosome
• Accession: NZ_CP081684
• Organism: Escherichia coli strain 0H23

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: J6O16_RS03830
• Protein ID: WP_000415584.1
• Coordinates: 775345..775641 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: J6O16_RS03835
• Protein ID: WP_000650107.1
• Coordinates: 775643..776038 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O16_RS03795 (770479)	770479..770958	+	480	WP_000065333.1	Hcp family type VI secretion system effector	-
J6O16_RS03800 (770961)	770961..771671	+	711	WP_000834029.1	hypothetical protein	-
J6O16_RS03805 (771678)	771678..772010	+	333	WP_000914691.1	DUF2645 family protein	-
J6O16_RS03810 (772056)	772056..773405	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
J6O16_RS03815 (773402)	773402..774061	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
J6O16_RS03820 (774213)	774213..774605	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
J6O16_RS03825 (774658)	774658..775140	+	483	WP_000609258.1	GyrI-like domain-containing protein	-
J6O16_RS03830 (775345)	775345..775641	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
J6O16_RS03835 (775643)	775643..776038	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
J6O16_RS03840 (776171)	776171..777778	+	1608	WP_104457550.1	ABC transporter substrate-binding protein	-
J6O16_RS03845 (777916)	777916..780174	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T213006 WP_000415584.1 NZ_CP081684:775345-775641 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T213006 NZ_CP107427:c149694-149203 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT213006 WP_000650107.1 NZ_CP081684:775643-776038 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT213006 NZ_CP107427:c149948-149682 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65