Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 708207..708934 Replicon chromosome
Accession NZ_CP081684
Organism Escherichia coli strain 0H23

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag J6O16_RS03510 Protein ID WP_000550189.1
Coordinates 708207..708521 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag J6O16_RS03515 Protein ID WP_000560266.1
Coordinates 708518..708934 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O16_RS03490 (704372) 704372..705358 - 987 WP_223584379.1 Gfo/Idh/MocA family oxidoreductase -
J6O16_RS03495 (705437) 705437..706120 - 684 WP_001183040.1 vancomycin high temperature exclusion protein -
J6O16_RS03500 (706197) 706197..706700 - 504 WP_001295542.1 M48 family metallopeptidase -
J6O16_RS03505 (706786) 706786..707922 + 1137 WP_000018697.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
J6O16_RS03510 (708207) 708207..708521 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
J6O16_RS03515 (708518) 708518..708934 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
J6O16_RS03520 (708979) 708979..710997 - 2019 WP_000121421.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
J6O16_RS03525 (711523) 711523..713874 - 2352 WP_000695498.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T213005 WP_000550189.1 NZ_CP081684:708207-708521 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T213005 NZ_CP107427:c125825-125409 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT213005 WP_000560266.1 NZ_CP081684:708518-708934 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT213005 NZ_CP107427:c126052-125822 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References