212919

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4225158..4225699 Replicon chromosome
Accession NZ_CP081489
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag K5T46_RS20335 Protein ID WP_000615981.1
Coordinates 4225158..4225436 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag K5T46_RS20340 Protein ID WP_000729704.1
Coordinates 4225439..4225699 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5T46_RS20320 (4222740) 4222740..4223318 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
K5T46_RS20325 (4223524) 4223524..4224291 + 768 WP_000333379.1 class II glutamine amidotransferase -
K5T46_RS20330 (4224262) 4224262..4225002 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K5T46_RS20335 (4225158) 4225158..4225436 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K5T46_RS20340 (4225439) 4225439..4225699 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K5T46_RS20345 (4225885) 4225885..4226658 + 774 WP_001353765.1 C40 family peptidase -
K5T46_RS20350 (4226834) 4226834..4227331 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
K5T46_RS20355 (4227547) 4227547..4229259 - 1713 Protein_4014 flagellar biosynthesis protein FlhA -
K5T46_RS20360 (4229231) 4229231..4230016 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4216655..4233319 16664


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T212919 WP_000615981.1 NZ_CP081489:c4225436-4225158 [Escherichia coli BL21(DE3)]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T212919 NZ_CP107403:1461091-1461309 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT212919 WP_000729704.1 NZ_CP081489:c4225699-4225439 [Escherichia coli BL21(DE3)]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT212919 NZ_CP107403:1460690-1461064 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References