Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 77284..77977 Replicon chromosome
Accession NZ_CP081489
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag K5T46_RS00350 Protein ID WP_000415584.1
Coordinates 77681..77977 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag K5T46_RS00345 Protein ID WP_000650107.1
Coordinates 77284..77679 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5T46_RS00335 (73148) 73148..75406 - 2259 WP_001281877.1 DNA topoisomerase IV subunit A -
K5T46_RS00340 (75544) 75544..77151 - 1608 WP_001295629.1 ABC transporter substrate-binding protein -
K5T46_RS00345 (77284) 77284..77679 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
K5T46_RS00350 (77681) 77681..77977 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
K5T46_RS00355 (78182) 78182..78664 - 483 WP_000183505.1 GyrI-like domain-containing protein -
K5T46_RS00360 (78717) 78717..79109 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
K5T46_RS00365 (79261) 79261..79920 + 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB -
K5T46_RS00370 (79917) 79917..81266 + 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
K5T46_RS00375 (81312) 81312..81644 - 333 WP_000917684.1 DUF2645 family protein -
K5T46_RS00380 (81963) 81963..82544 + 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
K5T46_RS00385 (82575) 82575..82889 + 315 WP_000958598.1 putative quinol monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T212901 WP_000415584.1 NZ_CP081489:c77977-77681 [Escherichia coli BL21(DE3)]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T212901 NZ_CP107398:289390-289758 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT212901 WP_000650107.1 NZ_CP081489:c77679-77284 [Escherichia coli BL21(DE3)]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT212901 NZ_CP107398:289172-289393 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References