Detailed information of TA system TA212901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 77284..77977
• Replicon: chromosome
• Accession: NZ_CP081489
• Organism: Escherichia coli BL21(DE3)

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: K5T46_RS00350
• Protein ID: WP_000415584.1
• Coordinates: 77681..77977 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: K5T46_RS00345
• Protein ID: WP_000650107.1
• Coordinates: 77284..77679 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5T46_RS00335 (73148)	73148..75406	-	2259	WP_001281877.1	DNA topoisomerase IV subunit A	-
K5T46_RS00340 (75544)	75544..77151	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
K5T46_RS00345 (77284)	77284..77679	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
K5T46_RS00350 (77681)	77681..77977	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
K5T46_RS00355 (78182)	78182..78664	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
K5T46_RS00360 (78717)	78717..79109	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
K5T46_RS00365 (79261)	79261..79920	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
K5T46_RS00370 (79917)	79917..81266	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
K5T46_RS00375 (81312)	81312..81644	-	333	WP_000917684.1	DUF2645 family protein	-
K5T46_RS00380 (81963)	81963..82544	+	582	WP_000072420.1	NADPH:quinone oxidoreductase MdaB	-
K5T46_RS00385 (82575)	82575..82889	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T212901 WP_000415584.1 NZ_CP081489:c77977-77681 [Escherichia coli BL21(DE3)]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T212901 NZ_CP107398:289390-289758 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT212901 WP_000650107.1 NZ_CP081489:c77679-77284 [Escherichia coli BL21(DE3)]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT212901 NZ_CP107398:289172-289393 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65