Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 40577..41227 Replicon chromosome
Accession NZ_CP081351
Organism Klebsiella michiganensis strain CCRI-24235

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag Km24235_RS00195 Protein ID WP_014227314.1
Coordinates 40577..40918 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag Km24235_RS00200 Protein ID WP_014227313.1
Coordinates 40928..41227 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Km24235_RS00180 (Km24235_0036) 36569..37888 + 1320 WP_032751824.1 MFS transporter -
Km24235_RS00185 (Km24235_0037) 38034..39425 + 1392 WP_222582123.1 hexose-6-phosphate:phosphate antiporter -
Km24235_RS00190 (Km24235_0038) 39874..40326 + 453 WP_032718748.1 DUF1198 domain-containing protein -
Km24235_RS00195 (Km24235_0039) 40577..40918 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
Km24235_RS00200 (Km24235_0040) 40928..41227 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
Km24235_RS00205 (Km24235_0041) 41346..42527 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
Km24235_RS00210 (Km24235_0042) 42560..43369 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
Km24235_RS00215 (Km24235_0043) 43379..44482 - 1104 WP_222582124.1 2-aminoethylphosphonate--pyruvate transaminase -
Km24235_RS00220 (Km24235_0044) 44623..45342 + 720 WP_222582125.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T212846 WP_014227314.1 NZ_CP081351:40577-40918 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T212846 NZ_CP107379:4181239-4181457 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT212846 WP_014227313.1 NZ_CP081351:40928-41227 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT212846 NZ_CP107379:4180838-4181212 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References