Detailed information of TA system TA212843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5677224..5677819
• Replicon: chromosome
• Accession: NZ_CP081346
• Organism: Pseudomonas aeruginosa strain SE5419

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: K5B69_RS26815
• Protein ID: WP_003113526.1
• Coordinates: 5677541..5677819 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5B69_RS26810
• Protein ID: WP_003113527.1
• Coordinates: 5677224..5677529 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B69_RS26790 (K5B69_26790)	5672617..5672889	-	273	WP_003115921.1	hypothetical protein	-
K5B69_RS26795 (K5B69_26795)	5672999..5673265	+	267	WP_023088595.1	hypothetical protein	-
K5B69_RS26800 (K5B69_26800)	5673321..5673656	+	336	WP_079862847.1	hypothetical protein	-
K5B69_RS26805 (K5B69_26805)	5673771..5676230	-	2460	WP_124089962.1	SIR2 family protein	-
K5B69_RS26810 (K5B69_26810)	5677224..5677529	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
K5B69_RS26815 (K5B69_26815)	5677541..5677819	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5B69_RS35325	5677872..5678000	-	129	Protein_5317	integrase	-
K5B69_RS26820 (K5B69_26820)	5678148..5680376	+	2229	WP_028680758.1	TonB-dependent receptor	-
K5B69_RS26825 (K5B69_26825)	5680446..5681093	-	648	WP_003095021.1	carbonate dehydratase	-
K5B69_RS26830 (K5B69_26830)	5681155..5682393	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T212843 WP_003113526.1 NZ_CP081346:c5677819-5677541 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T212843 NZ_CP107379:871659-872066 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT212843 WP_003113527.1 NZ_CP081346:c5677529-5677224 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT212843 NZ_CP107379:871412-871678 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3