Detailed information of TA system TA212836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5850915..5851510
• Replicon: chromosome
• Accession: NZ_CP081345
• Organism: Pseudomonas aeruginosa strain F291007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: K5B02_RS27355
• Protein ID: WP_003113526.1
• Coordinates: 5851232..5851510 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5B02_RS27350
• Protein ID: WP_003133769.1
• Coordinates: 5850915..5851220 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B02_RS27335 (K5B02_27335)	5847621..5848484	-	864	WP_031761182.1	integrase domain-containing protein	-
K5B02_RS27340 (K5B02_27340)	5849085..5850242	-	1158	WP_031761183.1	STY4528 family pathogenicity island replication protein	-
K5B02_RS27350 (K5B02_27350)	5850915..5851220	-	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
K5B02_RS27355 (K5B02_27355)	5851232..5851510	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5B02_RS32350	5851563..5851691	-	129	Protein_5419	integrase	-
K5B02_RS27360 (K5B02_27360)	5851839..5854067	+	2229	WP_023104079.1	TonB-dependent receptor	-
K5B02_RS27365 (K5B02_27365)	5854138..5854785	-	648	WP_003095021.1	carbonate dehydratase	-
K5B02_RS27370 (K5B02_27370)	5854847..5856085	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T212836 WP_003113526.1 NZ_CP081345:c5851510-5851232 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T212836 NZ_CP107373:c78125-77709 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT212836 WP_003133769.1 NZ_CP081345:c5851220-5850915 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT212836 NZ_CP107373:c78352-78122 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3