Detailed information of TA system TA212797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 63499..64211
• Replicon: plasmid pEc1079_2
• Accession: NZ_CP081308
• Organism: Escherichia coli strain Ec1079

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: K5A55_RS24245
• Protein ID: WP_000162415.1
• Coordinates: 63909..64211 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5A55_RS24240
• Protein ID: WP_032220986.1
• Coordinates: 63499..63837 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5A55_RS24210 (K5A55_24210)	58793..59614	-	822	WP_001427910.1	hypothetical protein	-
K5A55_RS24215 (K5A55_24215)	59961..60614	+	654	WP_221582558.1	maturation control protein	-
K5A55_RS24220 (K5A55_24220)	60943..61272	+	330	WP_000542383.1	hypothetical protein	-
K5A55_RS24225 (K5A55_24225)	61265..62458	+	1194	WP_097433648.1	phage tail protein	-
K5A55_RS24230 (K5A55_24230)	62492..63220	-	729	WP_032220985.1	hypothetical protein	-
K5A55_RS24235 (K5A55_24235)	63257..63442	-	186	Protein_81	hypothetical protein	-
K5A55_RS24240 (K5A55_24240)	63499..63837	-	339	WP_032220986.1	HigA family addiction module antitoxin	Antitoxin
K5A55_RS24245 (K5A55_24245)	63909..64211	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5A55_RS24250 (K5A55_24250)	64374..65165	+	792	WP_221582559.1	phage tail protein	-
K5A55_RS24255 (K5A55_24255)	65162..65929	+	768	WP_000203293.1	hypothetical protein	-
K5A55_RS24260 (K5A55_24260)	65933..66913	+	981	WP_000046500.1	hypothetical protein	-
K5A55_RS24265 (K5A55_24265)	66910..67563	+	654	WP_221582560.1	carbohydrate-binding domain-containing protein	-
K5A55_RS24270 (K5A55_24270)	67623..68528	+	906	WP_032220988.1	recombination-associated protein RdgC	-
K5A55_RS24275 (K5A55_24275)	68542..69192	+	651	WP_169781366.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90661	90661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T212797 WP_000162415.1 NZ_CP081308:c64211-63909 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T212797 NZ_CP107363:c2388066-2387698 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13068.01 Da
• Isoelectric Point: 6.4754

>AT212797 WP_032220986.1 NZ_CP081308:c63837-63499 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNMNKKAA

Nucleotide

• Length: 339 bp

>AT212797 NZ_CP107363:c2388284-2388063 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3