Detailed information of TA system TA212790

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58661..58900
• Replicon: plasmid pEc1079_1
• Accession: NZ_CP081307
• Organism: Escherichia coli strain Ec1079

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: K5A55_RS23605
• Protein ID: WP_023144756.1
• Coordinates: 58661..58795 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	58840..58900 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5A55_RS23575 (54362)	54362..55219	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
K5A55_RS23580 (55212)	55212..55286	-	75	WP_031943482.1	RepA leader peptide Tap	-
K5A55_RS23585 (55286)	55286..55417	-	132	Protein_69	protein CopA/IncA	-
K5A55_RS23590 (55648)	55648..57189	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
K5A55_RS23595 (57204)	57204..57950	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
K5A55_RS23600 (58110)	58110..58364	-	255	WP_000083850.1	replication regulatory protein RepA	-
K5A55_RS23605 (58661)	58661..58795	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (58840)	58840..58900	+	61	NuclAT_0	-	Antitoxin
- (58840)	58840..58900	+	61	NuclAT_0	-	Antitoxin
- (58840)	58840..58900	+	61	NuclAT_0	-	Antitoxin
- (58840)	58840..58900	+	61	NuclAT_0	-	Antitoxin
K5A55_RS23610 (58867)	58867..59153	-	287	Protein_74	DUF2726 domain-containing protein	-
K5A55_RS23615 (59666)	59666..59878	-	213	WP_013023861.1	hypothetical protein	-
K5A55_RS23620 (60009)	60009..60569	-	561	WP_000139341.1	fertility inhibition protein FinO	-
K5A55_RS23625 (60624)	60624..61370	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
K5A55_RS23630 (61390)	61390..63783	-	2394	Protein_78	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA5 / blaTEM-1B / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..98473	98473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T212790 WP_023144756.1 NZ_CP081307:c58795-58661 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T212790 NZ_CP107360:c79181-79032 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT212790 NZ_CP081307:58840-58900 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin