Detailed information of TA system TA212774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1111655..1112382
• Replicon: chromosome
• Accession: NZ_CP081306
• Organism: Escherichia coli strain Ec1079

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: K5A55_RS05430
• Protein ID: WP_000547564.1
• Coordinates: 1111655..1111966 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5A55_RS05435
• Protein ID: WP_000126294.1
• Coordinates: 1111963..1112382 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5A55_RS05400 (1106797)	1106797..1108506	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
K5A55_RS05405 (1108516)	1108516..1109058	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
K5A55_RS05410 (1109058)	1109058..1109825	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
K5A55_RS05415 (1109822)	1109822..1110232	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
K5A55_RS05420 (1110225)	1110225..1110695	+	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
K5A55_RS05425 (1110720)	1110720..1111481	+	762	WP_001026446.1	hypothetical protein	-
K5A55_RS05430 (1111655)	1111655..1111966	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
K5A55_RS05435 (1111963)	1111963..1112382	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
K5A55_RS05440 (1112496)	1112496..1113920	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
K5A55_RS05445 (1113929)	1113929..1115386	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
K5A55_RS05450 (1115646)	1115646..1116656	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
K5A55_RS05455 (1116805)	1116805..1117332	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T212774 WP_000547564.1 NZ_CP081306:1111655-1111966 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T212774 NZ_CP107353:217839-218282 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT212774 WP_000126294.1 NZ_CP081306:1111963-1112382 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT212774 NZ_CP107353:217612-217842 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5