Detailed information of TA system TA212762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5610800..5611395
• Replicon: chromosome
• Accession: NZ_CP081287
• Organism: Pseudomonas aeruginosa strain F092021

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: K3T18_RS26355
• Protein ID: WP_003117425.1
• Coordinates: 5611117..5611395 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K3T18_RS26350
• Protein ID: WP_003113527.1
• Coordinates: 5610800..5611105 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3T18_RS26315 (K3T18_26315)	5605940..5606788	+	849	WP_023122792.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
K3T18_RS26325 (K3T18_26325)	5606955..5607896	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
K3T18_RS26330 (K3T18_26330)	5608013..5608627	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
K3T18_RS26335 (K3T18_26335)	5608669..5609253	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
K3T18_RS26340 (K3T18_26340)	5609294..5610394	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
K3T18_RS26350 (K3T18_26350)	5610800..5611105	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
K3T18_RS26355 (K3T18_26355)	5611117..5611395	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K3T18_RS31745	5611448..5611576	-	129	Protein_5223	integrase	-
K3T18_RS26360 (K3T18_26360)	5611724..5613952	+	2229	WP_003099265.1	TonB-dependent receptor	-
K3T18_RS26365 (K3T18_26365)	5614022..5614669	-	648	WP_003095021.1	carbonate dehydratase	-
K3T18_RS26370 (K3T18_26370)	5614731..5615969	-	1239	WP_031685043.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T212762 WP_003117425.1 NZ_CP081287:c5611395-5611117 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T212762 NZ_CP107352:1358924-1359142 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT212762 WP_003113527.1 NZ_CP081287:c5611105-5610800 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT212762 NZ_CP107352:1358523-1358897 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5