Detailed information of TA system TA212739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4612174..4612772
• Replicon: chromosome
• Accession: NZ_CP081201
• Organism: Pseudomonas phytophila strain ICMP 23753

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: K3169_RS20850
• Protein ID: WP_231673658.1
• Coordinates: 4612174..4612452 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K3169_RS20855
• Protein ID: WP_027898900.1
• Coordinates: 4612461..4612772 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3169_RS20820 (K3169_20680)	4607828..4608637	+	810	WP_263267801.1	hypothetical protein	-
K3169_RS20825 (K3169_20685)	4608703..4609611	-	909	WP_122706019.1	50S ribosomal protein L3 N(5)-glutamine methyltransferase	-
K3169_RS20830 (K3169_20690)	4609872..4610462	+	591	WP_027898896.1	cysteine hydrolase family protein	-
K3169_RS20835 (K3169_20695)	4610697..4611017	+	321	WP_027898897.1	hypothetical protein	-
K3169_RS20840 (K3169_20700)	4611206..4611763	+	558	WP_263267802.1	Smr/MutS family protein	-
K3169_RS20845 (K3169_20705)	4611794..4611982	-	189	WP_122395631.1	hypothetical protein	-
K3169_RS20850 (K3169_20710)	4612174..4612452	+	279	WP_231673658.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K3169_RS20855 (K3169_20715)	4612461..4612772	+	312	WP_027898900.1	HigA family addiction module antitoxin	Antitoxin
K3169_RS20860 (K3169_20720)	4613049..4613594	+	546	WP_027898901.1	GTP cyclohydrolase I FolE	-
K3169_RS20865 (K3169_20725)	4613671..4614042	+	372	WP_027898902.1	glutathione S-transferase N-terminal domain-containing protein	-
K3169_RS20870 (K3169_20730)	4614064..4614456	-	393	WP_231677550.1	lysozyme inhibitor LprI family protein	-
K3169_RS20875 (K3169_20735)	4614628..4615230	-	603	WP_099267310.1	glutathione S-transferase family protein	-
K3169_RS20880 (K3169_20740)	4615264..4616430	-	1167	WP_231677551.1	PLP-dependent aminotransferase family protein	-
K3169_RS20885 (K3169_20745)	4616513..4616983	+	471	WP_231677552.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10506.95 Da
• Isoelectric Point: 7.9160

>T212739 WP_231673658.1 NZ_CP081201:4612174-4612452 [Pseudomonas phytophila]
MIQSFRCIDTQALFETGKTRRWSGLKNVAERKLAMLDAATALKDLKSPPGNRLEELHGDREGQHSIRINAQFRVCFLWGS
NGPEHVEIVDYH

Nucleotide

• Length: 279 bp

>T212739 NZ_CP107342:c58398-57982 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11481.20 Da
• Isoelectric Point: 6.4765

>AT212739 WP_027898900.1 NZ_CP081201:4612461-4612772 [Pseudomonas phytophila]
MHKNGMRPIHPGEILKEEFLEPLGVTPAALARALHVSAPTVNDIVRERRNISADVALRLATALGTSAQFWLNLQASYDLR
KAEIEKGEEIYRDVHLLDCCAFS

Nucleotide

• Length: 312 bp

>AT212739 NZ_CP107342:c58625-58395 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA