Detailed information of TA system TA021272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 316192..316796
• Replicon: chromosome
• Accession: NC_007492
• Organism: Pseudomonas fluorescens Pf0-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3KJP2
• Locus tag: PFL01_RS01365
• Protein ID: WP_011331970.1
• Coordinates: 316482..316796 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PFL01_RS01360
• Protein ID: WP_041475108.1
• Coordinates: 316192..316479 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFL01_RS01335	311226..312005	-	780	WP_011331965.1	EAL domain-containing protein	-
PFL01_RS01340	312122..312529	+	408	WP_011331966.1	RNA-binding protein S4	-
PFL01_RS01345	312596..313396	-	801	WP_011331967.1	phosphatase PAP2 family protein	-
PFL01_RS01350	313501..314403	+	903	WP_011331968.1	Hsp33 family molecular chaperone HslO	-
PFL01_RS01355	314585..316126	+	1542	WP_007952682.1	phosphoenolpyruvate carboxykinase	-
PFL01_RS01360	316192..316479	-	288	WP_041475108.1	helix-turn-helix domain-containing protein	Antitoxin
PFL01_RS01365	316482..316796	-	315	WP_011331970.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PFL01_RS01370	316908..317207	-	300	WP_011331971.1	hypothetical protein	-
PFL01_RS01375	317758..318885	-	1128	WP_011331972.1	hypothetical protein	-
PFL01_RS01380	318988..319269	-	282	WP_011331973.1	hypothetical protein	-
PFL01_RS01385	319573..320076	+	504	WP_011331974.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11847.76 Da
• Isoelectric Point: 10.3534

>T21272 WP_011331970.1 NC_007492:c316796-316482 [Pseudomonas fluorescens Pf0-1]
MIFIETRIFTRRVKELLDDDTYAAFQKQLVVSPSIGDVIEGTGGIRKTRIAAKGHGKRGGARVIYYHFVSASQIGLLMIY
PKNEQHDLSSDERKALKVLIEKWR

Nucleotide

• Length: 315 bp

>T21272 NC_007492:c316796-316482 [Pseudomonas fluorescens Pf0-1]
ATGATTTTTATTGAGACTCGGATCTTTACCCGTCGAGTAAAAGAGCTGCTGGACGATGACACTTACGCCGCTTTTCAGAA
GCAACTGGTTGTAAGTCCTTCGATTGGCGATGTAATTGAAGGCACAGGCGGGATCCGAAAAACCCGAATCGCAGCGAAAG
GACACGGCAAGCGAGGCGGAGCACGGGTTATCTACTACCACTTCGTTTCGGCATCACAAATCGGGTTATTGATGATCTAC
CCTAAAAATGAACAACACGATCTGTCCAGCGATGAGCGTAAAGCACTCAAGGTCTTGATCGAAAAATGGAGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10747.32 Da
• Isoelectric Point: 6.8814

>AT21272 WP_041475108.1 NC_007492:c316479-316192 [Pseudomonas fluorescens Pf0-1]
MSKFFDELMESVQEMDEIVRGERTPSREFHVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAH

Nucleotide

• Length: 288 bp

>AT21272 NC_007492:c316479-316192 [Pseudomonas fluorescens Pf0-1]
ATGAGCAAATTTTTTGATGAGCTGATGGAAAGTGTGCAAGAGATGGACGAGATCGTACGAGGCGAGCGCACACCTTCTCG
CGAATTCCACGTTGATGCATTGCAGGTCAAAGAAATTCGTAAAGCTACCGGTTTGACCCAGGCCAAATTTGCGGCACTGA
TCGACGTCCAGCTCGGCACTCTACGAAATTGGGAACAGGGTCGTCGCGAGCCAACCGGGCCGGCAAAAGCTCTGCTCAAG
GCCATTCATAACGACCCGGTTCATGTTTTGAATGCATTGGCCCATTGA

Structures

Toxin

• AlphaFold DB: Q3KJP2