Detailed information of TA system TA212702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1888469..1888971
• Replicon: chromosome
• Accession: NZ_CP081192
• Organism: Escherichia coli strain EC1722-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: K4F91_RS09160
• Protein ID: WP_000767829.1
• Coordinates: 1888717..1888971 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: K4F91_RS09155
• Protein ID: WP_001259255.1
• Coordinates: 1888469..1888720 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4F91_RS09130 (1883647)	1883647..1884714	-	1068	WP_000080141.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
K4F91_RS09135 (1884714)	1884714..1885784	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
K4F91_RS09140 (1885781)	1885781..1887085	-	1305	WP_001367667.1	histidinol dehydrogenase	-
K4F91_RS09145 (1887091)	1887091..1887990	-	900	WP_000131792.1	ATP phosphoribosyltransferase	-
K4F91_RS09150 (1888136)	1888136..1888186	-	51	WP_001364200.1	his operon leader peptide	-
K4F91_RS09155 (1888469)	1888469..1888720	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
K4F91_RS09160 (1888717)	1888717..1888971	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
K4F91_RS09165 (1889054)	1889054..1889878	+	825	WP_000754737.1	SDR family oxidoreductase	-
K4F91_RS09170 (1889924)	1889924..1890853	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
K4F91_RS09175 (1891068)	1891068..1891130	+	63	WP_010723108.1	membrane protein YoeI	-
K4F91_RS09180 (1891120)	1891120..1892478	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
K4F91_RS09185 (1892657)	1892657..1893715	+	1059	WP_000492333.1	thiosulfate utilization transporter TsuA/YeeE	-
K4F91_RS09190 (1893729)	1893729..1893956	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T212702 WP_000767829.1 NZ_CP081192:1888717-1888971 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T212702 NZ_CP107327:c5364457-5364074 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT212702 WP_001259255.1 NZ_CP081192:1888469-1888720 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT212702 NZ_CP107327:c5364699-5364457 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0