Detailed information of TA system TA212700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1076322..1077049
• Replicon: chromosome
• Accession: NZ_CP081192
• Organism: Escherichia coli strain EC1722-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: K4F91_RS05245
• Protein ID: WP_000547564.1
• Coordinates: 1076322..1076633 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K4F91_RS05250
• Protein ID: WP_000126294.1
• Coordinates: 1076630..1077049 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4F91_RS05215 (1071464)	1071464..1073173	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
K4F91_RS05220 (1073183)	1073183..1073725	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
K4F91_RS05225 (1073725)	1073725..1074492	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
K4F91_RS05230 (1074489)	1074489..1074899	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
K4F91_RS05235 (1074892)	1074892..1075362	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
K4F91_RS05240 (1075387)	1075387..1076148	+	762	WP_001026446.1	hypothetical protein	-
K4F91_RS05245 (1076322)	1076322..1076633	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
K4F91_RS05250 (1076630)	1076630..1077049	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
K4F91_RS05255 (1077163)	1077163..1078587	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
K4F91_RS05260 (1078596)	1078596..1080053	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
K4F91_RS05265 (1080313)	1080313..1081323	+	1011	WP_001363554.1	DNA-binding transcriptional regulator AscG	-
K4F91_RS05270 (1081472)	1081472..1081999	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T212700 WP_000547564.1 NZ_CP081192:1076322-1076633 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T212700 NZ_CP107327:c4884123-4883839 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT212700 WP_000126294.1 NZ_CP081192:1076630-1077049 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT212700 NZ_CP107327:c4884355-4884113 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5