Detailed information of TA system TA021270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3134886..3135479
• Replicon: chromosome
• Accession: NC_007484
• Organism: Nitrosococcus oceani ATCC 19707

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3J7I6
• Locus tag: NOC_RS14660
• Protein ID: WP_002814243.1
• Coordinates: 3134886..3135164 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NOC_RS14665
• Protein ID: WP_002814171.1
• Coordinates: 3135177..3135479 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOC_RS14635	3130446..3131852	-	1407	WP_002812363.1	mercury(II) reductase	-
NOC_RS14640	3131842..3132102	-	261	WP_002813332.1	mercury resistance system transport protein MerF	-
NOC_RS14645	3132209..3132616	+	408	WP_002811971.1	Hg(II)-responsive transcriptional regulator	-
NOC_RS14650	3132954..3134252	+	1299	Protein_2884	glycosyltransferase	-
NOC_RS14660	3134886..3135164	+	279	WP_002814243.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NOC_RS14665	3135177..3135479	+	303	WP_002814171.1	HigA family addiction module antidote protein	Antitoxin
NOC_RS14670	3135502..3135859	-	358	Protein_2887	transposase	-
NOC_RS14675	3135929..3138184	-	2256	WP_002814158.1	tetratricopeptide repeat protein	-
NOC_RS14680	3138261..3139313	-	1053	WP_002812282.1	DNA-binding protein	-
NOC_RS14685	3139657..3140121	-	465	WP_002812288.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-92)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10747.26 Da
• Isoelectric Point: 8.7536

>T21270 WP_002814243.1 NC_007484:3134886-3135164 [Nitrosococcus oceani ATCC 19707]
MIKCFRCKETQRLFETGKSRRWQNIRAVAERKLAQINAARELRDLASPPGNQLEALEGDRAGQYGIRINKQWRVCFIWTD
EGPEAVEIVDYH

Nucleotide

• Length: 279 bp

>T21270 NC_007484:3134886-3135164 [Nitrosococcus oceani ATCC 19707]
ATGATCAAGTGCTTCCGCTGCAAAGAAACCCAACGGTTATTTGAAACTGGAAAATCCCGCCGGTGGCAAAACATCCGCGC
GGTAGCCGAACGTAAGCTAGCCCAGATAAACGCTGCACGGGAACTGCGAGATCTCGCCTCCCCGCCAGGCAACCAACTGG
AAGCCTTAGAAGGCGACCGCGCCGGACAATACGGTATTCGTATCAACAAGCAATGGCGGGTGTGCTTTATCTGGACGGAC
GAGGGACCGGAAGCCGTTGAAATTGTAGACTATCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11685.51 Da
• Isoelectric Point: 8.6404

>AT21270 WP_002814171.1 NC_007484:3135177-3135479 [Nitrosococcus oceani ATCC 19707]
MFKNRMRPVHPGEILREDYLKPLEMSVNALAKALHAPTPRINDVVLERRGVTADTAMRLARYFDTTPQFWMTLQMEHDLR
VAEIERANRIESEVQPRQVA

Nucleotide

• Length: 303 bp

>AT21270 NC_007484:3135177-3135479 [Nitrosococcus oceani ATCC 19707]
ATGTTTAAAAACAGGATGCGCCCGGTTCACCCGGGCGAAATTTTGCGCGAGGACTACCTAAAACCATTGGAAATGAGCGT
CAATGCACTGGCCAAGGCATTGCATGCACCCACGCCCCGCATCAACGATGTCGTACTCGAGCGGCGCGGCGTAACGGCGG
ACACGGCGATGCGCCTGGCGCGCTATTTTGATACGACACCCCAATTTTGGATGACCTTGCAAATGGAGCACGATTTGCGT
GTAGCTGAAATTGAGCGGGCAAACCGGATAGAAAGCGAAGTTCAGCCGCGTCAAGTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0E2Z4E1