212696

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 603347..604146 Replicon chromosome
Accession NZ_CP081192
Organism Escherichia coli strain EC1722-1

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag K4F91_RS02945 Protein ID WP_000347251.1
Coordinates 603347..603811 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K4F91_RS02950 Protein ID WP_001307405.1
Coordinates 603811..604146 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K4F91_RS02915 (598348) 598348..598782 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K4F91_RS02920 (598800) 598800..599678 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K4F91_RS02925 (599668) 599668..600447 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K4F91_RS02930 (600458) 600458..600931 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K4F91_RS02935 (600954) 600954..602234 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
K4F91_RS02940 (602483) 602483..603292 + 810 WP_000072192.1 aga operon transcriptional regulator AgaR -
K4F91_RS02945 (603347) 603347..603811 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K4F91_RS02950 (603811) 603811..604146 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K4F91_RS02955 (604295) 604295..605866 - 1572 WP_001273761.1 galactarate dehydratase -
K4F91_RS02960 (606241) 606241..607575 + 1335 WP_000599653.1 galactarate/glucarate/glycerate transporter GarP -
K4F91_RS02965 (607591) 607591..608361 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T212696 WP_000347251.1 NZ_CP081192:c603811-603347 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T212696 NZ_CP107327:2009758-2009860 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT212696 WP_001307405.1 NZ_CP081192:c604146-603811 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT212696 NZ_CP107327:c2009867-2009722 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CJ20


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References