Detailed information of TA system TA212580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 21777..22423
• Replicon: plasmid p25POL-A
• Accession: NZ_CP081011
• Organism: Escherichia coli strain 25POL

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7R6ZH56
• Locus tag: K3072_RS24790
• Protein ID: WP_000269913.1
• Coordinates: 22076..22423 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1V3UZT0
• Locus tag: K3072_RS24785
• Protein ID: WP_001259436.1
• Coordinates: 21777..22076 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3072_RS24755 (K3072_24755)	17611..18183	-	573	WP_000137931.1	helix-turn-helix transcriptional regulator	-
K3072_RS24760 (K3072_24760)	18757..18948	+	192	WP_000183352.1	hypothetical protein	-
K3072_RS24765 (K3072_24765)	18945..20087	+	1143	WP_000854799.1	ORF6N domain-containing protein	-
K3072_RS24770 (K3072_24770)	20098..20286	-	189	WP_000986262.1	hypothetical protein	-
K3072_RS24775 (K3072_24775)	20897..21169	-	273	WP_000148349.1	helix-turn-helix domain-containing protein	-
K3072_RS24780 (K3072_24780)	21233..21613	-	381	WP_000061764.1	hypothetical protein	-
K3072_RS24785 (K3072_24785)	21777..22076	-	300	WP_001259436.1	XRE family transcriptional regulator	Antitoxin
K3072_RS24790 (K3072_24790)	22076..22423	-	348	WP_000269913.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K3072_RS24795 (K3072_24795)	22668..22931	-	264	WP_000424604.1	hypothetical protein	-
K3072_RS24800 (K3072_24800)	22955..23242	-	288	WP_000356589.1	hypothetical protein	-
K3072_RS24805 (K3072_24805)	23886..24866	-	981	WP_228465520.1	plasmid replication initiator RepA	-
K3072_RS24810 (K3072_24810)	25048..25602	-	555	WP_001199265.1	recombinase family protein	-
K3072_RS24815 (K3072_24815)	25663..26631	+	969	Protein_42	phage tail protein	-
K3072_RS24820 (K3072_24820)	26634..27167	+	534	WP_000972173.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49741	49741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13486.53 Da
• Isoelectric Point: 9.8723

>T212580 WP_000269913.1 NZ_CP081011:c22423-22076 [Escherichia coli]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T212580 NZ_CP081011:c22423-22076 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11074.92 Da
• Isoelectric Point: 5.2090

>AT212580 WP_001259436.1 NZ_CP081011:c22076-21777 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT212580 NZ_CP081011:c22076-21777 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Structures

Toxin

• AlphaFold DB: A0A7R6ZH56

Antitoxin

• AlphaFold DB: A0A1V3UZT0