Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4914651..4915253 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag K3072_RS23580 Protein ID WP_000897302.1
Coordinates 4914942..4915253 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K3072_RS23575 Protein ID WP_000356397.1
Coordinates 4914651..4914941 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS23550 (4910724) 4910724..4911626 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
K3072_RS23555 (4911623) 4911623..4912258 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
K3072_RS23560 (4912255) 4912255..4913184 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
K3072_RS23565 (4913400) 4913400..4913618 - 219 WP_001314326.1 CopG family transcriptional regulator -
K3072_RS23570 (4914014) 4914014..4914292 - 279 WP_001296612.1 hypothetical protein -
K3072_RS23575 (4914651) 4914651..4914941 - 291 WP_000356397.1 NadS family protein Antitoxin
K3072_RS23580 (4914942) 4914942..4915253 - 312 WP_000897302.1 hypothetical protein Toxin
K3072_RS23585 (4915483) 4915483..4916391 + 909 WP_001305059.1 alpha/beta hydrolase -
K3072_RS23590 (4916455) 4916455..4917396 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
K3072_RS23595 (4917441) 4917441..4917878 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
K3072_RS23600 (4917875) 4917875..4918747 - 873 WP_000920762.1 virulence factor BrkB family protein -
K3072_RS23605 (4918741) 4918741..4919340 - 600 WP_001443191.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T212579 WP_000897302.1 NZ_CP081010:c4915253-4914942 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T212579 NZ_CP107284:2996932-2997588 [Escherichia coli]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGCAAAAAGCTCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGAAACGTGGCAGCACACGACGTACCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGGATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT212579 WP_000356397.1 NZ_CP081010:c4914941-4914651 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT212579 NZ_CP107284:2997585-2998652 [Escherichia coli]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTGAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATTTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCAAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References