Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1212891..1213618 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag K3072_RS05820 Protein ID WP_000547555.1
Coordinates 1212891..1213202 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag K3072_RS05825 Protein ID WP_000126293.1
Coordinates 1213199..1213618 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS05790 (1208032) 1208032..1209741 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
K3072_RS05795 (1209751) 1209751..1210293 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
K3072_RS05800 (1210293) 1210293..1211060 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
K3072_RS05805 (1211057) 1211057..1211467 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
K3072_RS05810 (1211460) 1211460..1211930 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
K3072_RS05815 (1211973) 1211973..1212728 + 756 WP_001305268.1 hypothetical protein -
K3072_RS05820 (1212891) 1212891..1213202 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
K3072_RS05825 (1213199) 1213199..1213618 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
K3072_RS05830 (1213710) 1213710..1214138 - 429 WP_000536064.1 hypothetical protein -
K3072_RS05835 (1214523) 1214523..1215050 + 528 WP_001078777.1 electron transport protein HydN -
K3072_RS05840 (1215203) 1215203..1217455 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T212556 WP_000547555.1 NZ_CP081010:1212891-1213202 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T212556 NZ_CP107272:c1112748-1112329 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT212556 WP_000126293.1 NZ_CP081010:1213199-1213618 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT212556 NZ_CP107272:c1112984-1112748 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References