Detailed information of TA system TA212553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 621236..622035
• Replicon: chromosome
• Accession: NZ_CP081010
• Organism: Escherichia coli strain 25POL

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: K3072_RS03045
• Protein ID: WP_000347252.1
• Coordinates: 621236..621700 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: K3072_RS03050
• Protein ID: WP_001296435.1
• Coordinates: 621700..622035 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3072_RS03015 (616237)	616237..616671	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
K3072_RS03020 (616689)	616689..617567	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
K3072_RS03025 (617557)	617557..618336	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
K3072_RS03030 (618347)	618347..618820	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
K3072_RS03035 (618843)	618843..620123	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
K3072_RS03040 (620372)	620372..621181	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
K3072_RS03045 (621236)	621236..621700	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
K3072_RS03050 (621700)	621700..622035	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
K3072_RS03055 (622184)	622184..623755	-	1572	WP_001273940.1	galactarate dehydratase	-
K3072_RS03060 (624130)	624130..625464	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
K3072_RS03065 (625480)	625480..626250	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T212553 WP_000347252.1 NZ_CP081010:c621700-621236 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T212553 NZ_CP107270:769100-769519 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT212553 WP_001296435.1 NZ_CP081010:c622035-621700 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT212553 NZ_CP107270:768864-769100 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5