Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4457748..4458289 Replicon chromosome
Accession NZ_CP081009
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag K3830_RS21620 Protein ID WP_000615983.1
Coordinates 4458011..4458289 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag K3830_RS21615 Protein ID WP_000729703.1
Coordinates 4457748..4458008 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3830_RS21595 (4452976) 4452976..4454391 - 1416 WP_000695655.1 glutamate--tRNA ligase -
K3830_RS21600 (4454705) 4454705..4455892 + 1188 Protein_4254 flagellar biosynthesis protein FlhA -
K3830_RS21605 (4456116) 4456116..4456613 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
K3830_RS21610 (4456789) 4456789..4457538 - 750 WP_000056849.1 C40 family peptidase -
K3830_RS21615 (4457748) 4457748..4458008 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K3830_RS21620 (4458011) 4458011..4458289 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K3830_RS21625 (4458445) 4458445..4459185 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K3830_RS21630 (4459156) 4459156..4459923 - 768 WP_000333380.1 class II glutamine amidotransferase -
K3830_RS21635 (4460118) 4460118..4460306 - 189 Protein_4261 GTP-binding protein -
K3830_RS21660 (4461224) 4461224..4462174 + 951 WP_000023081.1 type I pantothenate kinase -
K3830_RS21665 (4462203) 4462203..4463168 - 966 WP_000654630.1 bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T212549 WP_000615983.1 NZ_CP081009:4458011-4458289 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T212549 NZ_CP107267:2584-2862 [Neisseria gonorrhoeae]
ATGTACGCGATTTCTTTTGATTTGGTGGTTGCCGATACCGCTCAAAACCATCCGAAAGGCATTTCCCAAGCCTACGCGGA
TATTGGATACACCCTGCGGAAATTCGGCTTTACCCGCATTCAGGGAAGTTTGTACACCTGCCAAAACGAAGATATGGCTA
ACCTGTTTTCAGCCATCAACGAGCTAAAAGCTCTGCCTTGGTTTCCGTCTTCTGTCCGCGATATTCGGGCGTTCCGCATT
GAGCAGTGGTCTGATTTCACAAGTCTTGTGAAGTCTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT212549 WP_000729703.1 NZ_CP081009:4457748-4458008 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT212549 NZ_CP107267:2399-2584 [Neisseria gonorrhoeae]
ATGCAAACCACTATCCCCACCGGTTCAATCCGCACCTTTGGCGATTACGGCGTGATGTACATCGTCGGCACGCCTGCCGA
ACAGCTTCCGGACGGCGATTGGCTCGTAAATATTGAACTGCCGGAAAGCGGCGAACACACGCAATACAAACTGTCCCACA
TCGTTCAAGACCCAAAGGCTGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References