Detailed information of TA system TA212546

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 4444272..4444693
• Replicon: chromosome
• Accession: NZ_CP081009
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K3830_RS21535
• Protein ID: WP_096937776.1
• Coordinates: 4444272..4444397 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4444495..4444693 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3830_RS21490 (4439444)	4439444..4439749	+	306	WP_010892536.1	hypothetical protein	-
K3830_RS21495 (4439826)	4439826..4440509	+	684	WP_000085939.1	DNA methylase	-
K3830_RS21500 (4440510)	4440510..4440731	+	222	WP_001104904.1	hypothetical protein	-
K3830_RS21505 (4440625)	4440625..4441179	+	555	WP_001358893.1	DUF1380 family protein	-
K3830_RS21510 (4441225)	4441225..4442001	+	777	WP_001005032.1	hypothetical protein	-
K3830_RS21515 (4442415)	4442415..4442840	+	426	WP_001198920.1	antirestriction protein	-
K3830_RS21520 (4442887)	4442887..4443309	+	423	WP_000271685.1	DUF1380 family protein	-
K3830_RS21525 (4443306)	4443306..4443497	+	192	WP_001027495.1	hypothetical protein	-
K3830_RS22360 (4443823)	4443823..4443971	+	149	Protein_4241	pilus protein	-
K3830_RS21535 (4444272)	4444272..4444397	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K3830_RS22365 (4444339)	4444339..4444488	-	150	Protein_4243	DUF5431 family protein	-
- (4444495)	4444495..4444693	-	199	NuclAT_1	-	Antitoxin
- (4444495)	4444495..4444693	-	199	NuclAT_1	-	Antitoxin
- (4444495)	4444495..4444693	-	199	NuclAT_1	-	Antitoxin
- (4444495)	4444495..4444693	-	199	NuclAT_1	-	Antitoxin
K3830_RS21540 (4444662)	4444662..4445424	-	763	Protein_4244	plasmid SOS inhibition protein A	-
K3830_RS21545 (4445421)	4445421..4445855	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
K3830_RS21550 (4445910)	4445910..4447868	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
K3830_RS21555 (4447934)	4447934..4448167	-	234	WP_000005990.1	DUF905 family protein	-
K3830_RS21560 (4448224)	4448224..4448763	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
K3830_RS21565 (4449081)	4449081..4449278	+	198	Protein_4249	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T212546 WP_096937776.1 NZ_CP081009:c4444397-4444272 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T212546 NZ_CP107264:c1631420-1631238 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT212546 NZ_CP081009:c4444693-4444495 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin