Detailed information of TA system TA212544

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4304253..4304507
• Replicon: chromosome
• Accession: NZ_CP081009
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: K3830_RS20775
• Protein ID: WP_001312851.1
• Coordinates: 4304253..4304402 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	4304446..4304507 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3830_RS20755 (4300362)	4300362..4300913	+	552	WP_001235704.1	recombinase family protein	-
K3830_RS20760 (4300929)	4300929..4303025	+	2097	WP_000422420.1	hypothetical protein	-
K3830_RS20765 (4303404)	4303404..4303478	-	75	WP_001442103.1	RepA leader peptide Tap	-
K3830_RS20770 (4303712)	4303712..4303969	-	258	WP_000083834.1	replication regulatory protein RepA	-
K3830_RS20775 (4304253)	4304253..4304402	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (4304446)	4304446..4304507	+	62	NuclAT_37	-	Antitoxin
- (4304446)	4304446..4304507	+	62	NuclAT_37	-	Antitoxin
- (4304446)	4304446..4304507	+	62	NuclAT_37	-	Antitoxin
- (4304446)	4304446..4304507	+	62	NuclAT_37	-	Antitoxin
K3830_RS20780 (4304803)	4304803..4305114	+	312	WP_000802277.1	hypothetical protein	-
K3830_RS20785 (4305295)	4305295..4305381	-	87	Protein_4093	endonuclease	-
K3830_RS20790 (4305579)	4305579..4305767	-	189	WP_001345829.1	hypothetical protein	-
K3830_RS20795 (4305902)	4305902..4306069	-	168	Protein_4095	ProQ/FINO family protein	-
K3830_RS20800 (4306221)	4306221..4307096	-	876	Protein_4096	inverse autotransporter beta domain-containing protein	-
K3830_RS22350 (4307648)	4307648..4307737	+	90	Protein_4097	hypothetical protein	-
K3830_RS20805 (4308211)	4308211..4308312	+	102	WP_000662258.1	small membrane protein YkgR	-
K3830_RS20810 (4308676)	4308676..4308939	+	264	WP_000803998.1	type B 50S ribosomal protein L31	-
K3830_RS20815 (4308939)	4308939..4309079	+	141	WP_000866436.1	type B 50S ribosomal protein L36	-
K3830_RS20820 (4309114)	4309114..4309341	-	228	WP_001147279.1	protein YkgL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T212544 WP_001312851.1 NZ_CP081009:c4304402-4304253 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T212544 NZ_CP107263:70075-70359 [Leclercia adecarboxylata]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTGCAGATGCAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATACCGCCTTGTCTATCGTGTAGAAGACGACATTGTCACAGTCACTGTTATCGGAGTAGGCAAA
CGCGAAAACGACGACATTTATAATGTAACCCTGAATCGCAATTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT212544 NZ_CP081009:4304446-4304507 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin