Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4179547..4180241 Replicon chromosome
Accession NZ_CP081009
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag K3830_RS19975 Protein ID WP_001263489.1
Coordinates 4179843..4180241 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag K3830_RS19970 Protein ID WP_000554758.1
Coordinates 4179547..4179840 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3830_RS19955 (4177034) 4177034..4177612 - 579 Protein_3933 flagellar biosynthesis protein FlhA -
K3830_RS19960 (4177584) 4177584..4178369 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
K3830_RS19965 (4178440) 4178440..4179495 + 1056 WP_001226164.1 DNA polymerase IV -
K3830_RS19970 (4179547) 4179547..4179840 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
K3830_RS19975 (4179843) 4179843..4180241 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
K3830_RS19980 (4180251) 4180251..4180703 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
K3830_RS19985 (4181021) 4181021..4181227 + 207 Protein_3939 RtcB family protein -
K3830_RS19990 (4181223) 4181223..4181744 + 522 Protein_3940 peptide chain release factor H -
K3830_RS19995 (4181801) 4181801..4183258 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
K3830_RS20000 (4183519) 4183519..4183977 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4184573) 4184573..4184653 + 81 NuclAT_11 - -
- (4184573) 4184573..4184653 + 81 NuclAT_11 - -
- (4184573) 4184573..4184653 + 81 NuclAT_11 - -
- (4184573) 4184573..4184653 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T212541 WP_001263489.1 NZ_CP081009:4179843-4180241 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T212541 NZ_CP107262:c2605624-2605226 [Leclercia adecarboxylata]
ATGCTGCATATGCTGGACACTAACATAGTAAGCCATCTCGTGAGGCAGCATCCAGGAGTGGTAAATCACTATTCCCGGTT
GGCGCCAGAAGATATGTGTATTTCAAGCGTAGCGGAGGCTGAGCTGCTTTACGGCGTTGCCAAAAAGCAAAGTCTTAGAT
TGCACGAAACCATCTCGGAATTTCTTAAAACTATCACCGTTTGCGACTGGGACAGTGACGCCGCGGCAATATACGGCAAG
CTGCGCGCCGAAATGGAAAAGCGCGGGAAAGTCATGGGCGATCTCGATCAGTTGATCGCAGCGCACGCAATCAGCCGGGG
CACAACGATTGTGACTAACGATCAGGCTTTCGCCATGGTGCAGGAACTGACAGTCGAAGACTGGACTACCGCAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT212541 WP_000554758.1 NZ_CP081009:4179547-4179840 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT212541 NZ_CP107262:c2605888-2605628 [Leclercia adecarboxylata]
ATGGAACGTAAAGCAAAACTGTTCAAAAAAGGGCGAAACCAGGCGGTTTTACTGCCTGCTGAGTTCGCGTTTGATGCGGA
GAGTGTCTGGATCCGGCGGGATGAAGAGGGGAATGTTGTGTTGAGGGCTATGTCAGAGAAGGAACGGCACAGGGAAAATT
TTTTGCGTTTACTGAAGCAGACTCATGTCCCTGATTCTTTCCTGAGTAAAGAGGAACGCAATCAGAGCTATACGACAAGA
GATCCTTTTGAAGGATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References