Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2520304..2521031 Replicon chromosome
Accession NZ_CP081009
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag K3830_RS12025 Protein ID WP_000550189.1
Coordinates 2520304..2520618 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K3830_RS12030 Protein ID WP_000560266.1
Coordinates 2520615..2521031 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3830_RS12005 (2516462) 2516462..2517448 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
K3830_RS12010 (2517527) 2517527..2518219 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
K3830_RS12015 (2518296) 2518296..2518799 - 504 WP_001333820.1 M48 family metallopeptidase -
K3830_RS12020 (2518884) 2518884..2520020 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
K3830_RS12025 (2520304) 2520304..2520618 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
K3830_RS12030 (2520615) 2520615..2521031 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K3830_RS12035 (2521076) 2521076..2523094 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
K3830_RS12040 (2523520) 2523520..2525871 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T212529 WP_000550189.1 NZ_CP081009:2520304-2520618 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T212529 NZ_CP107257:2547639-2547926 [Pseudomonas aeruginosa]
GTGCGCGTACTTGAGTGGCGGGAAGCTGCCCGCGCCGACCTGCTGGCGATTGTTGACTACATCTCGGACGACAACCCCGA
TGCCGCGCAGCGGTTGAAGGACGACATCGAGGCCAAGGCCGCGAAGCTGCCGGAGCGCCCGAAGCTCTACCGGCCTGGCC
GCGTGGCCGGCACGCGGGAGATGGTCGTGCGTGCGAACTACGTGGTGGTCTACATGGAGGACACCCGCGCGGTGTCCATC
CTGCGCGTGTTGCACGCCGCGCAGCAGTGGCCGCCGGCACGGGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT212529 WP_000560266.1 NZ_CP081009:2520615-2521031 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT212529 NZ_CP107257:2547353-2547652 [Pseudomonas aeruginosa]
ATGGCCGCACAGACTTCGATGCTGCACATCCGCGTGGACGATAAGCTCAAGGCCGATGCAGCCGAGAAACTCGCCGGGGT
CGGCCTCACCGTTTCGGACGCCGTGCGCATCCTGCTGACCCGTGTGGTCAAGGAAGGCGCACTGCCGGCTGGCCTCACGG
CCGACCCGGAGGCCTACGATGCCTGGTTCCGGGCGAAGGTCCAGGAGGCGCTGGCCGACACACGGCCGCCGGTGCCGCAC
CAGCAGGTCATGGATGAGGCGCAGGCCCTGATCGACAGGAAGCGGCGTGCGCGTACTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References