Detailed information of TA system TA212504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3057461..3058155
• Replicon: chromosome
• Accession: NZ_CP081008
• Organism: Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: K3831_RS14545
• Protein ID: WP_001263489.1
• Coordinates: 3057461..3057859 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: K3831_RS14550
• Protein ID: WP_000554758.1
• Coordinates: 3057862..3058155 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3053049)	3053049..3053129	-	81	NuclAT_13	-	-
- (3053049)	3053049..3053129	-	81	NuclAT_13	-	-
- (3053049)	3053049..3053129	-	81	NuclAT_13	-	-
- (3053049)	3053049..3053129	-	81	NuclAT_13	-	-
K3831_RS14520 (3053725)	3053725..3054183	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
K3831_RS14525 (3054444)	3054444..3055901	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
K3831_RS14530 (3055958)	3055958..3056479	-	522	Protein_2870	peptide chain release factor H	-
K3831_RS14535 (3056475)	3056475..3056681	-	207	Protein_2871	RtcB family protein	-
K3831_RS14540 (3056999)	3056999..3057451	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
K3831_RS14545 (3057461)	3057461..3057859	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
K3831_RS14550 (3057862)	3057862..3058155	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
K3831_RS14555 (3058207)	3058207..3059262	-	1056	WP_001226164.1	DNA polymerase IV	-
K3831_RS14560 (3059333)	3059333..3060118	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
K3831_RS14565 (3060090)	3060090..3061802	+	1713	Protein_2877	flagellar biosynthesis protein FlhA	-
K3831_RS14570 (3062026)	3062026..3062523	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3030180..3076133	45953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T212504 WP_001263489.1 NZ_CP081008:c3057859-3057461 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T212504 NZ_CP107112:c2069505-2069215 [Clostridioides difficile]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT212504 WP_000554758.1 NZ_CP081008:c3058155-3057862 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT212504 NZ_CP107112:c2069780-2069502 [Clostridioides difficile]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3