Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2346077..2346804 Replicon chromosome
Accession NZ_CP081008
Organism Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag K3831_RS11160 Protein ID WP_000550189.1
Coordinates 2346490..2346804 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K3831_RS11155 Protein ID WP_000560266.1
Coordinates 2346077..2346493 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3831_RS11145 (2341237) 2341237..2343588 + 2352 WP_000695487.1 alpha-glucosidase -
K3831_RS11150 (2344014) 2344014..2346032 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
K3831_RS11155 (2346077) 2346077..2346493 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K3831_RS11160 (2346490) 2346490..2346804 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
K3831_RS11165 (2347088) 2347088..2348224 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
K3831_RS11170 (2348309) 2348309..2348812 + 504 WP_001333820.1 M48 family metallopeptidase -
K3831_RS11175 (2348889) 2348889..2349581 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
K3831_RS11180 (2349660) 2349660..2350646 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T212496 WP_000550189.1 NZ_CP081008:c2346804-2346490 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T212496 NZ_CP107077:c4612519-4612151 [Kosakonia cowanii]
ATGAGCAACCTTGCGTTATTTGATACCAATATCTTGATTGATCTGTTTAATGGCCGGGAAGAGGCTGCTAACACTTTTGA
AGATTACAATGGCAGGATCGCCATCAGCCTGATTAGCTGGATTGAAGTGCTGGTTGGCGCTAAAAAAGTAGGGCAGGAAA
ATGCGACACGCGATTTTCTTGATTCGCTTGAGGTCATAGAGATTTCCCATCCCATAGCAGAACGAAGCATTGAGCTGCGT
CAGGCGTATCGCATGAAGCTGCCCGATGCCATTATTCTTGCAACAGCTCAGATACATAGCCGTTGCCTCGTTACGCGCAA
TATCAAAGATTTTGCGTCTGTAACAGGGGTTTTCACCCCATATCAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT212496 WP_000560266.1 NZ_CP081008:c2346493-2346077 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT212496 NZ_CP107077:c4612764-4612522 [Kosakonia cowanii]
ATGGATGTTCTCTCTGGAGTGATTATGGGGCGTATTTTGTTGGATTTATCAGATGATGTTATACGCAGGCTCGATAGCTT
AAAGCAGCAGCGTAACCTTCCTCGTGCTGAATTATTACGAGAAGCGGTTGAGCAGTATCTTGAAAAGCAGAACACGACAA
GCGCTCGCAATGCTTTAGGTCTATGGGGAGGTGAGGGCGAAGATGGTCTTGAATTTGAGAGAAAACTGCGCGAGGAGTGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References