Detailed information of TA system TA212474

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 4572071..4572492
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K3826_RS22125
• Protein ID: WP_096937776.1
• Coordinates: 4572071..4572196 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4572294..4572492 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS22080 (4567120)	4567120..4567425	+	306	WP_010892536.1	hypothetical protein	-
K3826_RS22085 (4567502)	4567502..4568185	+	684	WP_000085939.1	DNA methylase	-
K3826_RS22090 (4568186)	4568186..4568407	+	222	WP_001104904.1	hypothetical protein	-
K3826_RS22095 (4568301)	4568301..4568855	+	555	WP_001358893.1	DUF1380 family protein	-
K3826_RS22100 (4568901)	4568901..4569677	+	777	WP_001005032.1	hypothetical protein	-
K3826_RS22105 (4570091)	4570091..4570516	+	426	WP_001198920.1	antirestriction protein	-
K3826_RS22110 (4570563)	4570563..4570985	+	423	WP_000271685.1	DUF1380 family protein	-
K3826_RS22115 (4570982)	4570982..4571173	+	192	WP_001027495.1	hypothetical protein	-
K3826_RS22895 (4571502)	4571502..4571770	+	269	Protein_4355	hypothetical protein	-
K3826_RS22125 (4572071)	4572071..4572196	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
K3826_RS22900 (4572138)	4572138..4572287	-	150	Protein_4357	DUF5431 family protein	-
- (4572294)	4572294..4572492	-	199	NuclAT_1	-	Antitoxin
- (4572294)	4572294..4572492	-	199	NuclAT_1	-	Antitoxin
- (4572294)	4572294..4572492	-	199	NuclAT_1	-	Antitoxin
- (4572294)	4572294..4572492	-	199	NuclAT_1	-	Antitoxin
K3826_RS22130 (4572461)	4572461..4573223	-	763	Protein_4358	plasmid SOS inhibition protein A	-
K3826_RS22135 (4573220)	4573220..4573654	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
K3826_RS22140 (4573709)	4573709..4575667	-	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
K3826_RS22145 (4575733)	4575733..4575966	-	234	WP_000005990.1	DUF905 family protein	-
K3826_RS22150 (4576023)	4576023..4576562	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
K3826_RS22155 (4576880)	4576880..4577104	+	225	Protein_4363	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T212474 WP_096937776.1 NZ_CP081007:c4572196-4572071 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T212474 NZ_CP107043:3646334-3646723 [Klebsiella pneumoniae]
ATGCAAACCAAATCATCCCCCCTCATGCGGGCGGCATCGTCACATCCGTCTCCCGTGGGTGTCTGGCAGACTCTGCTGAC
CAGCCTGCTTGAACACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGCGATGAACAGGTGATTCAGCAGCATATCG
AGGCGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAAAAGTATGAACTGGTCCGGACTGACCGGCAAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGAAAGGCTACAGGGCTGATGAACCTTGG
CACGTATAAAGACGTGACGGCTATTACCAAAGGACAACATCAGCAGGCCAACACTTCAGGCAAGCGATGA

Antitoxin

Nucleotide

• Length: 199 bp

>AT212474 NZ_CP081007:c4572492-4572294 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin