Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4072548..4073089 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag K3826_RS19535 Protein ID WP_000615983.1
Coordinates 4072811..4073089 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag K3826_RS19530 Protein ID WP_000729703.1
Coordinates 4072548..4072808 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3826_RS19510 (4068223) 4068223..4069008 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
K3826_RS19515 (4068980) 4068980..4070692 + 1713 Protein_3829 flagellar biosynthesis protein FlhA -
K3826_RS19520 (4070916) 4070916..4071413 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
K3826_RS19525 (4071589) 4071589..4072338 - 750 WP_000056849.1 C40 family peptidase -
K3826_RS19530 (4072548) 4072548..4072808 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
K3826_RS19535 (4072811) 4072811..4073089 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
K3826_RS19540 (4073245) 4073245..4073985 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
K3826_RS19545 (4073956) 4073956..4074723 - 768 WP_000333380.1 class II glutamine amidotransferase -
K3826_RS19550 (4074998) 4074998..4075537 - 540 WP_001096705.1 DUF2058 domain-containing protein -
K3826_RS19555 (4075639) 4075639..4076850 - 1212 WP_000107627.1 3-(3-hydroxy-phenyl)propionate transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4057288..4164989 107701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T212467 WP_000615983.1 NZ_CP081007:4072811-4073089 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T212467 NZ_CP107043:1493170-1493388 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT212467 WP_000729703.1 NZ_CP081007:4072548-4072808 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT212467 NZ_CP107043:1492769-1493143 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References