Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4066351..4067045 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag K3826_RS19495 Protein ID WP_001263489.1
Coordinates 4066351..4066749 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag K3826_RS19500 Protein ID WP_000554758.1
Coordinates 4066752..4067045 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4061939) 4061939..4062019 - 81 NuclAT_13 - -
- (4061939) 4061939..4062019 - 81 NuclAT_13 - -
- (4061939) 4061939..4062019 - 81 NuclAT_13 - -
- (4061939) 4061939..4062019 - 81 NuclAT_13 - -
K3826_RS19470 (4062615) 4062615..4063073 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
K3826_RS19475 (4063334) 4063334..4064791 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
K3826_RS19480 (4064848) 4064848..4065369 - 522 Protein_3822 peptide chain release factor H -
K3826_RS19485 (4065365) 4065365..4065571 - 207 Protein_3823 RtcB family protein -
K3826_RS19490 (4065889) 4065889..4066341 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
K3826_RS19495 (4066351) 4066351..4066749 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
K3826_RS19500 (4066752) 4066752..4067045 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
K3826_RS19505 (4067097) 4067097..4068152 - 1056 WP_001226164.1 DNA polymerase IV -
K3826_RS19510 (4068223) 4068223..4069008 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
K3826_RS19515 (4068980) 4068980..4070692 + 1713 Protein_3829 flagellar biosynthesis protein FlhA -
K3826_RS19520 (4070916) 4070916..4071413 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4057288..4164989 107701


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T212466 WP_001263489.1 NZ_CP081007:c4066749-4066351 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T212466 NZ_CP107043:c186165-184843 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGATAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCACTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGTCGCCACGCCGACCACGCATATCATTAAGTTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGCGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCTATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGACTGCAGGCGCTATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT212466 WP_000554758.1 NZ_CP081007:c4067045-4066752 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT212466 NZ_CP107043:c186431-186165 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References