Detailed information of TA system TA021246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3003765..3004492
• Replicon: chromosome
• Accession: NC_007384
• Organism: Shigella sonnei Ss046

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: SSON_RS15855
• Protein ID: WP_000547563.1
• Coordinates: 3004181..3004492 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SSON_RS15850
• Protein ID: WP_000126296.1
• Coordinates: 3003765..3004184 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSON_RS15830 (2998816)	2998816..2999343	-	528	WP_001078766.1	electron transport protein HydN	-
SSON_RS15835 (2999491)	2999491..3000501	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
SSON_RS15840 (3000761)	3000761..3002218	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
SSON_RS15845 (3002227)	3002227..3003651	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
SSON_RS15850 (3003765)	3003765..3004184	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
SSON_RS15855 (3004181)	3004181..3004492	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
SSON_RS15860 (3004654)	3004654..3005427	-	774	WP_001026459.1	hypothetical protein	-
SSON_RS15865 (3005452)	3005452..3005922	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
SSON_RS15870 (3005915)	3005915..3006325	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
SSON_RS15875 (3006322)	3006322..3007089	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
SSON_RS15880 (3007089)	3007089..3007631	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
SSON_RS15885 (3007641)	3007641..3009338	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T21246 WP_000547563.1 NC_007384:c3004492-3004181 [Shigella sonnei Ss046]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T21246 NC_007384:c3004492-3004181 [Shigella sonnei Ss046]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT21246 WP_000126296.1 NC_007384:c3004184-3003765 [Shigella sonnei Ss046]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT21246 NC_007384:c3004184-3003765 [Shigella sonnei Ss046]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22